The AcMNPV bacmid was evolved for 5 passages in Spodoptera exigua larvae, amplified in S. exigua larvae, and then deep sequenced. The goal of the project was to use the bacterial derived sequences of the bacmid to estimate the baculovirus mutation rate, as mutations in these sequences can be assumed to not have effects of viral fitness.
Boezen, D., Ali, G., Wang, M., Wang, X., Werf, W. V. D., Vlak, J. M. & Zwart, M. P., 2022, In: PLoS Genetics.18, 6, e1009806.
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Boezen, D. (Creator), Zwart, M. (Creator) (22 Jan 2022). Autographa californica multiple nucleopolyhedrovirus bacmid Raw sequence reads . European Nucleotide Archive (ENA).