Plant genotypes can select beneficial rhizosphere microbiome to interact. However the success of this interaction is dependent of a series of factors that directly or indirectly influences the plant rhizosphere microbiome composition. In this study, we evaluate the rhizosphere bacterial community composition of seven different Sorghum bicolor (L.) Moench genotypes in four different growth stages cultivated in two different soil types. Our results showed that comparing soil and plant genotype effects along the plant growth, at the earlier stages (days 10 and 20), the Sorghum rhizosphere bacterial community composition was mainly driven by soil type whereas in later stages (days 35 and 50) the selection was also driven by the different Sorghum genotypes. This results point towards a successional process where the influence of Sorghum genotypes increases in time. Although cultivars had little effect on sorghum rhizosphere bacterial community composition, cultivar C6 at CF soil had significantly higher relative abundance of Acidobacteria GP1, Burkholderia, Cupriavidus (Burkholderiaceae), Acidovorax and Albidiferax (Comamonadaceae) compared with the other six cultivars. Even with a small effect of sorghum cultivars, our results showed that different sorghum cultivars have the capacity to differentially form it owns bacterial community composition. This was the first work to investigate the contribution of soil type, plant growth stages and plant genotype shaping the sorghum rhizosphere bacterial community composition.