α-catenin cytomechanics--role in cadherin-dependent adhesion and mechanotransduction

Adrienne K Barry, Hamid Tabdili, Ismaeel Muhamed, Jun Wu, Nitesh Shashikanth, Guillermo A Gomez, Alpha S Yap, Cara J Gottardi, Johan de Rooij, Ning Wang, Deborah E Leckband

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

The findings presented here demonstrate the role of α-catenin in cadherin-based adhesion and mechanotransduction in different mechanical contexts. Bead-twisting measurements in conjunction with imaging, and the use of different cell lines and α-catenin mutants reveal that the acute local mechanical manipulation of cadherin bonds triggers vinculin and actin recruitment to cadherin adhesions in an actin- and α-catenin-dependent manner. The modest effect of α-catenin on the two-dimensional binding affinities of cell surface cadherins further suggests that force-activated adhesion strengthening is due to enhanced cadherin-cytoskeletal interactions rather than to α-catenin-dependent affinity modulation. Complementary investigations of cadherin-based rigidity sensing also suggest that, although α-catenin alters traction force generation, it is not the sole regulator of cell contractility on compliant cadherin-coated substrata.

Original languageEnglish
Pages (from-to)1779-91
Number of pages13
JournalJournal of Cell Science
Volume127
Issue numberPt 8
DOIs
Publication statusPublished - 15 Apr 2014

Keywords

  • Actins
  • Animals
  • Binding Sites
  • Biomechanical Phenomena
  • Cadherins
  • Cell Adhesion
  • Cell Line, Tumor
  • Dogs
  • Erythrocytes
  • Humans
  • Kinetics
  • Madin Darby Canine Kidney Cells
  • Mechanotransduction, Cellular
  • Protein Interaction Domains and Motifs
  • Protein Transport
  • Vinculin
  • alpha Catenin

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