α-catenin cytomechanics--role in cadherin-dependent adhesion and mechanotransduction

Adrienne K Barry; Hamid Tabdili; Ismaeel Muhamed; Jun Wu; Nitesh Shashikanth; Guillermo A Gomez; Alpha S Yap; Cara J Gottardi; Johan de Rooij; Ning Wang; Deborah E Leckband

doi:10.1242/jcs.139014

α-catenin cytomechanics--role in cadherin-dependent adhesion and mechanotransduction

Adrienne K Barry, Hamid Tabdili, Ismaeel Muhamed, Jun Wu, Nitesh Shashikanth, Guillermo A Gomez, Alpha S Yap, Cara J Gottardi, Johan de Rooij, Ning Wang, Deborah E Leckband

Hubrecht Institute

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

95 Citations (Scopus)

Abstract

The findings presented here demonstrate the role of α-catenin in cadherin-based adhesion and mechanotransduction in different mechanical contexts. Bead-twisting measurements in conjunction with imaging, and the use of different cell lines and α-catenin mutants reveal that the acute local mechanical manipulation of cadherin bonds triggers vinculin and actin recruitment to cadherin adhesions in an actin- and α-catenin-dependent manner. The modest effect of α-catenin on the two-dimensional binding affinities of cell surface cadherins further suggests that force-activated adhesion strengthening is due to enhanced cadherin-cytoskeletal interactions rather than to α-catenin-dependent affinity modulation. Complementary investigations of cadherin-based rigidity sensing also suggest that, although α-catenin alters traction force generation, it is not the sole regulator of cell contractility on compliant cadherin-coated substrata.

Original language	English
Pages (from-to)	1779-91
Number of pages	13
Journal	Journal of Cell Science
Volume	127
Issue number	Pt 8
DOIs	https://doi.org/10.1242/jcs.139014
Publication status	Published - 15 Apr 2014

Keywords

Actins
Animals
Binding Sites
Biomechanical Phenomena
Cadherins
Cell Adhesion
Cell Line, Tumor
Dogs
Erythrocytes
Humans
Kinetics
Madin Darby Canine Kidney Cells
Mechanotransduction, Cellular
Protein Interaction Domains and Motifs
Protein Transport
Vinculin
alpha Catenin

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10.1242/jcs.139014

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title = "α-catenin cytomechanics--role in cadherin-dependent adhesion and mechanotransduction",

abstract = "The findings presented here demonstrate the role of α-catenin in cadherin-based adhesion and mechanotransduction in different mechanical contexts. Bead-twisting measurements in conjunction with imaging, and the use of different cell lines and α-catenin mutants reveal that the acute local mechanical manipulation of cadherin bonds triggers vinculin and actin recruitment to cadherin adhesions in an actin- and α-catenin-dependent manner. The modest effect of α-catenin on the two-dimensional binding affinities of cell surface cadherins further suggests that force-activated adhesion strengthening is due to enhanced cadherin-cytoskeletal interactions rather than to α-catenin-dependent affinity modulation. Complementary investigations of cadherin-based rigidity sensing also suggest that, although α-catenin alters traction force generation, it is not the sole regulator of cell contractility on compliant cadherin-coated substrata.",

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author = "Barry, {Adrienne K} and Hamid Tabdili and Ismaeel Muhamed and Jun Wu and Nitesh Shashikanth and Gomez, {Guillermo A} and Yap, {Alpha S} and Gottardi, {Cara J} and {de Rooij}, Johan and Ning Wang and Leckband, {Deborah E}",

year = "2014",

month = apr,

day = "15",

doi = "10.1242/jcs.139014",

language = "English",

volume = "127",

pages = "1779--91",

journal = "Journal of Cell Science",

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T1 - α-catenin cytomechanics--role in cadherin-dependent adhesion and mechanotransduction

AU - Barry, Adrienne K

AU - Tabdili, Hamid

AU - Muhamed, Ismaeel

AU - Wu, Jun

AU - Shashikanth, Nitesh

AU - Gomez, Guillermo A

AU - Yap, Alpha S

AU - Gottardi, Cara J

AU - de Rooij, Johan

AU - Wang, Ning

AU - Leckband, Deborah E

PY - 2014/4/15

Y1 - 2014/4/15

N2 - The findings presented here demonstrate the role of α-catenin in cadherin-based adhesion and mechanotransduction in different mechanical contexts. Bead-twisting measurements in conjunction with imaging, and the use of different cell lines and α-catenin mutants reveal that the acute local mechanical manipulation of cadherin bonds triggers vinculin and actin recruitment to cadherin adhesions in an actin- and α-catenin-dependent manner. The modest effect of α-catenin on the two-dimensional binding affinities of cell surface cadherins further suggests that force-activated adhesion strengthening is due to enhanced cadherin-cytoskeletal interactions rather than to α-catenin-dependent affinity modulation. Complementary investigations of cadherin-based rigidity sensing also suggest that, although α-catenin alters traction force generation, it is not the sole regulator of cell contractility on compliant cadherin-coated substrata.

AB - The findings presented here demonstrate the role of α-catenin in cadherin-based adhesion and mechanotransduction in different mechanical contexts. Bead-twisting measurements in conjunction with imaging, and the use of different cell lines and α-catenin mutants reveal that the acute local mechanical manipulation of cadherin bonds triggers vinculin and actin recruitment to cadherin adhesions in an actin- and α-catenin-dependent manner. The modest effect of α-catenin on the two-dimensional binding affinities of cell surface cadherins further suggests that force-activated adhesion strengthening is due to enhanced cadherin-cytoskeletal interactions rather than to α-catenin-dependent affinity modulation. Complementary investigations of cadherin-based rigidity sensing also suggest that, although α-catenin alters traction force generation, it is not the sole regulator of cell contractility on compliant cadherin-coated substrata.

KW - Actins

KW - Animals

KW - Binding Sites

KW - Biomechanical Phenomena

KW - Cadherins

KW - Cell Adhesion

KW - Cell Line, Tumor

KW - Dogs

KW - Erythrocytes

KW - Humans

KW - Kinetics

KW - Madin Darby Canine Kidney Cells

KW - Mechanotransduction, Cellular

KW - Protein Interaction Domains and Motifs

KW - Protein Transport

KW - Vinculin

KW - alpha Catenin

U2 - 10.1242/jcs.139014

DO - 10.1242/jcs.139014

M3 - Article

C2 - 24522187

SN - 0021-9533

VL - 127

SP - 1779

EP - 1791

JO - Journal of Cell Science

JF - Journal of Cell Science

IS - Pt 8

ER -

α-catenin cytomechanics--role in cadherin-dependent adhesion and mechanotransduction

Abstract

Keywords

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