1-Octen-3-ol inhibits conidia germination of Penicillium paneum despite of mild effects on membrane permeability, respiration, intracellular pH, and changes the protein composition

Gilma S Chitarra, Tjakko Abee, Frank M Rombouts, Jan Dijksterhuis

    Research output: Contribution to journal/periodicalArticleScientificpeer-review

    Abstract

    1-Octen-3-ol is a volatile germination self-inhibitor produced by Penicillium paneum that blocks the germination process. The size of conidia treated with 1-octen-3-ol was similar to that of freshly harvested conidia. Exposure to 1-octen-3-ol resulted in staining of 10-20% of the conidia with PI and TOTO, fluorescent DNA probes that cannot enter cells with an intact membrane, whereas only 3-5% of non-treated conidia were stained. Addition of 1-octen-3-ol to germinating conidia resulted in transient dissipation of the pH gradient. From this, we conclude that slight permeabilisation of the fungal membrane occurs in the presence of the inhibitor. Two-dimensional gel electrophoresis analysis of protein patterns revealed striking differences between non-germinated conidia, germinated conidia and 1-octen-3-ol-treated conidia. In conclusion, 1-octen-3-ol has mild effects on the plasma membrane, but interferes with essential metabolic processes, such as swelling and germination of the conidia, but in a reversible manner.

    Original languageEnglish
    Pages (from-to)67-75
    Number of pages9
    JournalFEMS Microbiology Ecology
    Volume54
    Issue number1
    DOIs
    Publication statusPublished - 01 Sep 2005

    Keywords

    • Cell Membrane Permeability
    • Fungal Proteins
    • Hydrogen-Ion Concentration
    • Octanols
    • Oxygen Consumption
    • Penicillium
    • Spores, Fungal

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