3D gastruloids: a novel frontier in stem cell-based in vitro modeling of mammalian gastrulation

Susanne C van den Brink; Alexander van Oudenaarden

doi:10.1016/j.tcb.2021.06.007

3D gastruloids: a novel frontier in stem cell-based in vitro modeling of mammalian gastrulation

Susanne C van den Brink, Alexander van Oudenaarden

Hubrecht Institute

Research output: Contribution to journal/periodical › Book/Film/Article review › Scientific

56 Citations (Scopus)

Abstract

3D gastruloids, aggregates of embryonic stem cells that recapitulate key aspects of gastrula-stage embryos, have emerged as a powerful tool to study the early stages of mammalian post-implantation development in vitro. Owing to their tractable nature and the relative ease by which they can be generated in large numbers, 3D gastruloids provide an unparalleled opportunity to study normal and pathological embryogenesis from a bottom-up perspective and in a high-throughput manner. Here, we review how gastruloid models can be exploited to deepen our understanding of mammalian development. In addition, we discuss current limitations, potential clinical applications, and ethical implications of this emerging model system.

Original language	English
Pages (from-to)	747-759
Number of pages	13
Journal	Trends in Cell Biology
Volume	31
Issue number	9
DOIs	https://doi.org/10.1016/j.tcb.2021.06.007
Publication status	Published - Sept 2021

Keywords

Animals
Embryonic Development
Embryonic Stem Cells
Gastrula
Gastrulation
Models, Biological

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10.1016/j.tcb.2021.06.007

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title = "3D gastruloids: a novel frontier in stem cell-based in vitro modeling of mammalian gastrulation",

abstract = "3D gastruloids, aggregates of embryonic stem cells that recapitulate key aspects of gastrula-stage embryos, have emerged as a powerful tool to study the early stages of mammalian post-implantation development in vitro. Owing to their tractable nature and the relative ease by which they can be generated in large numbers, 3D gastruloids provide an unparalleled opportunity to study normal and pathological embryogenesis from a bottom-up perspective and in a high-throughput manner. Here, we review how gastruloid models can be exploited to deepen our understanding of mammalian development. In addition, we discuss current limitations, potential clinical applications, and ethical implications of this emerging model system.",

keywords = "Animals, Embryonic Development, Embryonic Stem Cells, Gastrula, Gastrulation, Models, Biological",

author = "{van den Brink}, {Susanne C} and {van Oudenaarden}, Alexander",

year = "2021",

month = sep,

doi = "10.1016/j.tcb.2021.06.007",

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journal = "Trends in Cell Biology",

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T2 - a novel frontier in stem cell-based in vitro modeling of mammalian gastrulation

AU - van den Brink, Susanne C

AU - van Oudenaarden, Alexander

PY - 2021/9

Y1 - 2021/9

N2 - 3D gastruloids, aggregates of embryonic stem cells that recapitulate key aspects of gastrula-stage embryos, have emerged as a powerful tool to study the early stages of mammalian post-implantation development in vitro. Owing to their tractable nature and the relative ease by which they can be generated in large numbers, 3D gastruloids provide an unparalleled opportunity to study normal and pathological embryogenesis from a bottom-up perspective and in a high-throughput manner. Here, we review how gastruloid models can be exploited to deepen our understanding of mammalian development. In addition, we discuss current limitations, potential clinical applications, and ethical implications of this emerging model system.

AB - 3D gastruloids, aggregates of embryonic stem cells that recapitulate key aspects of gastrula-stage embryos, have emerged as a powerful tool to study the early stages of mammalian post-implantation development in vitro. Owing to their tractable nature and the relative ease by which they can be generated in large numbers, 3D gastruloids provide an unparalleled opportunity to study normal and pathological embryogenesis from a bottom-up perspective and in a high-throughput manner. Here, we review how gastruloid models can be exploited to deepen our understanding of mammalian development. In addition, we discuss current limitations, potential clinical applications, and ethical implications of this emerging model system.

KW - Animals

KW - Embryonic Development

KW - Embryonic Stem Cells

KW - Gastrula

KW - Gastrulation

KW - Models, Biological

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DO - 10.1016/j.tcb.2021.06.007

M3 - Book/Film/Article review

C2 - 34304959

SN - 0962-8924

VL - 31

SP - 747

EP - 759

JO - Trends in Cell Biology

JF - Trends in Cell Biology

IS - 9

ER -

3D gastruloids: a novel frontier in stem cell-based in vitro modeling of mammalian gastrulation

Abstract

Keywords

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