A common genetic variant within SCN10A modulates cardiac SCN5A expression

Malou van den Boogaard, Scott Smemo, Ozanna Burnicka-Turek, David E Arnolds, Harmen J G van de Werken, Petra Klous, David McKean, Jochen D Muehlschlegel, Julia Moosmann, Okan Toka, Xinan H Yang, Tamara T Koopmann, Michiel E Adriaens, Connie R Bezzina, Wouter de Laat, Christine Seidman, J G Seidman, Vincent M Christoffels, Marcelo A Nobrega, Phil BarnettIvan P Moskowitz

Research output: Contribution to journal/periodicalArticleScientificpeer-review

219 Downloads (Pure)

Abstract

Variants in SCN10A, which encodes a voltage-gated sodium channel, are associated with alterations of cardiac conduction parameters and the cardiac rhythm disorder Brugada syndrome; however, it is unclear how SCN10A variants promote dysfunctional cardiac conduction. Here we showed by high-resolution 4C-seq analysis of the Scn10a-Scn5a locus in murine heart tissue that a cardiac enhancer located in Scn10a, encompassing SCN10A functional variant rs6801957, interacts with the promoter of Scn5a, a sodium channel-encoding gene that is critical for cardiac conduction. We observed that SCN5A transcript levels were several orders of magnitude higher than SCN10A transcript levels in both adult human and mouse heart tissue. Analysis of BAC transgenic mouse strains harboring an engineered deletion of the enhancer within Scn10a revealed that the enhancer was essential for Scn5a expression in cardiac tissue. Furthermore, the common SCN10A variant rs6801957 modulated Scn5a expression in the heart. In humans, the SCN10A variant rs6801957, which correlated with slowed conduction, was associated with reduced SCN5A expression. These observations establish a genomic mechanism for how a common genetic variation at SCN10A influences cardiac physiology and predisposes to arrhythmia.

Original languageEnglish
Pages (from-to)1844-52
Number of pages9
JournalJournal of Clinical Investigation
Volume124
Issue number4
DOIs
Publication statusPublished - 01 Apr 2014

Keywords

  • Adult
  • Animals
  • Arrhythmias, Cardiac
  • Enhancer Elements, Genetic
  • Female
  • Gene Expression Regulation
  • Genetic Variation
  • Heart
  • Humans
  • Male
  • Mice
  • Mice, Transgenic
  • Myocardium
  • NAV1.5 Voltage-Gated Sodium Channel
  • NAV1.8 Voltage-Gated Sodium Channel
  • Polymorphism, Single Nucleotide
  • Pregnancy
  • Promoter Regions, Genetic
  • RNA, Messenger

Fingerprint Dive into the research topics of 'A common genetic variant within SCN10A modulates cardiac SCN5A expression'. Together they form a unique fingerprint.

  • Cite this

    van den Boogaard, M., Smemo, S., Burnicka-Turek, O., Arnolds, D. E., van de Werken, H. J. G., Klous, P., McKean, D., Muehlschlegel, J. D., Moosmann, J., Toka, O., Yang, X. H., Koopmann, T. T., Adriaens, M. E., Bezzina, C. R., de Laat, W., Seidman, C., Seidman, J. G., Christoffels, V. M., Nobrega, M. A., ... Moskowitz, I. P. (2014). A common genetic variant within SCN10A modulates cardiac SCN5A expression. Journal of Clinical Investigation, 124(4), 1844-52. https://doi.org/10.1172/JCI73140