A Comparison of Isolation Methods for Black Fungi Degrading Aromatic Toxins

Yu Quan, Bert Gerrits van den Ende, Dongmei Shi, Francesc X Prenafeta-Boldú, Zuoyi Liu, Abdullah M S Al-Hatmi, Sarah A Ahmed, Paul E Verweij, Yingqian Kang, Sybren de Hoog

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

The prevalence of black fungi in the order Chaetothyriales has often been underestimated due to the difficulty of their isolation. In this study, three methods which are often used to isolate black fungi are compared. Enrichment on aromatic hydrocarbon appears effective in inhibiting growth of cosmopolitan microbial species and allows appearance of black fungi. We miniaturized the method for high-throughput purposes. The new procedure saves time, consumes less space and can process multiple samples simultaneously.

Original languageEnglish
Pages (from-to)653-660
Number of pages8
JournalMycopathologia
Volume184
Issue number5
DOIs
Publication statusPublished - Oct 2019

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    Quan, Y., van den Ende, B. G., Shi, D., Prenafeta-Boldú, F. X., Liu, Z., Al-Hatmi, A. M. S., Ahmed, S. A., Verweij, P. E., Kang, Y., & de Hoog, S. (2019). A Comparison of Isolation Methods for Black Fungi Degrading Aromatic Toxins. Mycopathologia, 184(5), 653-660. https://doi.org/10.1007/s11046-019-00382-3