A decade of 3C technologies: insights into nuclear organization

E. de Wit; W. de Laat

doi:10.1101/gad.179804.111

A decade of 3C technologies: insights into nuclear organization

E. de Wit, W. de Laat

Hubrecht Institute

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

Abstract

Over the past 10 years, the development of chromosome conformation capture (3C) technology and the subsequent genomic variants thereof have enabled the analysis of nuclear organization at an unprecedented resolution and throughput. The technology relies on the original and, in hindsight, remarkably simple idea that digestion and religation of fixed chromatin in cells, followed by the quantification of ligation junctions, allows for the determination of DNA contact frequencies and insight into chromosome topology. Here we evaluate and compare the current 3C-based methods (including 4C [chromosome conformation capture-on-chip], 5C [chromosome conformation capture carbon copy], HiC, and ChIA-PET), summarize their contribution to our current understanding of genome structure, and discuss how shape influences genome function.

Original language	English
Pages (from-to)	11-24
Journal	Genes & Development
Volume	26
Issue number	1
DOIs	https://doi.org/10.1101/gad.179804.111
Publication status	Published - 2012

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