A Geographically Diverse Collection of Schizosaccharomyces pombe Isolates Shows Limited Phenotypic Variation but Extensive Karyotypic Diversity

William R A Brown, Gianni Liti, Carlos Rosa, Steve James, Ian Roberts, Vincent Robert, Neil Jolly, Wen Tang, Peter Baumann, Carter Green, Kristina Schlegel, Jonathan Young, Fabienne Hirchaud, Spencer Leek, Geraint Thomas, Anders Blomberg, Jonas Warringer

    Research output: Contribution to journal/periodicalArticleScientificpeer-review

    Abstract

    The fission yeast Schizosaccharomyces pombe has been widely used to study eukaryotic cell biology, but almost all of this work has used derivatives of a single strain. We have studied 81 independent natural isolates and 3 designated laboratory strains of Schizosaccharomyces pombe. Schizosaccharomyces pombe varies significantly in size but shows only limited variation in proliferation in different environments compared with Saccharomyces cerevisiae. Nucleotide diversity, π, at a near neutral site, the central core of the centromere of chromosome II is approximately 0.7%. Approximately 20% of the isolates showed karyotypic rearrangements as detected by pulsed field gel electrophoresis and filter hybridization analysis. One translocation, found in 6 different isolates, including the type strain, has a geographically widespread distribution and a unique haplotype and may be a marker of an incipient speciation event. All of the other translocations are unique. Exploitation of this karyotypic diversity may cast new light on both the biology of telomeres and centromeres and on isolating mechanisms in single-celled eukaryotes.

    Original languageEnglish
    Pages (from-to)615-26
    Number of pages12
    JournalG3: Genes, Genomes, Genetics
    Volume1
    Issue number7
    DOIs
    Publication statusPublished - Dec 2011

    Fingerprint Dive into the research topics of 'A Geographically Diverse Collection of Schizosaccharomyces pombe Isolates Shows Limited Phenotypic Variation but Extensive Karyotypic Diversity'. Together they form a unique fingerprint.

    Cite this