A Geographically Diverse Collection of Schizosaccharomyces pombe Isolates Shows Limited Phenotypic Variation but Extensive Karyotypic Diversity

William R A Brown; Gianni Liti; Carlos Rosa; Steve James; Ian Roberts; Vincent Robert; Neil Jolly; Wen Tang; Peter Baumann; Carter Green; Kristina Schlegel; Jonathan Young; Fabienne Hirchaud; Spencer Leek; Geraint Thomas; Anders Blomberg; Jonas Warringer

doi:10.1534/g3.111.001123

A Geographically Diverse Collection of Schizosaccharomyces pombe Isolates Shows Limited Phenotypic Variation but Extensive Karyotypic Diversity

William R A Brown, Gianni Liti, Carlos Rosa, Steve James, Ian Roberts, Vincent Robert, Neil Jolly, Wen Tang, Peter Baumann, Carter Green, Kristina Schlegel, Jonathan Young, Fabienne Hirchaud, Spencer Leek, Geraint Thomas, Anders Blomberg, Jonas Warringer

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

Abstract

The fission yeast Schizosaccharomyces pombe has been widely used to study eukaryotic cell biology, but almost all of this work has used derivatives of a single strain. We have studied 81 independent natural isolates and 3 designated laboratory strains of Schizosaccharomyces pombe. Schizosaccharomyces pombe varies significantly in size but shows only limited variation in proliferation in different environments compared with Saccharomyces cerevisiae. Nucleotide diversity, π, at a near neutral site, the central core of the centromere of chromosome II is approximately 0.7%. Approximately 20% of the isolates showed karyotypic rearrangements as detected by pulsed field gel electrophoresis and filter hybridization analysis. One translocation, found in 6 different isolates, including the type strain, has a geographically widespread distribution and a unique haplotype and may be a marker of an incipient speciation event. All of the other translocations are unique. Exploitation of this karyotypic diversity may cast new light on both the biology of telomeres and centromeres and on isolating mechanisms in single-celled eukaryotes.

Original language	English
Pages (from-to)	615-26
Number of pages	12
Journal	G3: Genes, Genomes, Genetics
Volume	1
Issue number	7
DOIs	https://doi.org/10.1534/g3.111.001123
Publication status	Published - Dec 2011

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Brown, W. R. A., Liti, G., Rosa, C., James, S., Roberts, I., Robert, V., Jolly, N., Tang, W., Baumann, P., Green, C., Schlegel, K., Young, J., Hirchaud, F., Leek, S., Thomas, G., Blomberg, A., & Warringer, J. (2011). A Geographically Diverse Collection of Schizosaccharomyces pombe Isolates Shows Limited Phenotypic Variation but Extensive Karyotypic Diversity. G3: Genes, Genomes, Genetics, 1(7), 615-26. https://doi.org/10.1534/g3.111.001123

Brown, William R A ; Liti, Gianni ; Rosa, Carlos ; James, Steve ; Roberts, Ian ; Robert, Vincent ; Jolly, Neil ; Tang, Wen ; Baumann, Peter ; Green, Carter ; Schlegel, Kristina ; Young, Jonathan ; Hirchaud, Fabienne ; Leek, Spencer ; Thomas, Geraint ; Blomberg, Anders ; Warringer, Jonas. / A Geographically Diverse Collection of Schizosaccharomyces pombe Isolates Shows Limited Phenotypic Variation but Extensive Karyotypic Diversity. In: G3: Genes, Genomes, Genetics. 2011 ; Vol. 1, No. 7. pp. 615-26.

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title = "A Geographically Diverse Collection of Schizosaccharomyces pombe Isolates Shows Limited Phenotypic Variation but Extensive Karyotypic Diversity",

abstract = "The fission yeast Schizosaccharomyces pombe has been widely used to study eukaryotic cell biology, but almost all of this work has used derivatives of a single strain. We have studied 81 independent natural isolates and 3 designated laboratory strains of Schizosaccharomyces pombe. Schizosaccharomyces pombe varies significantly in size but shows only limited variation in proliferation in different environments compared with Saccharomyces cerevisiae. Nucleotide diversity, π, at a near neutral site, the central core of the centromere of chromosome II is approximately 0.7%. Approximately 20% of the isolates showed karyotypic rearrangements as detected by pulsed field gel electrophoresis and filter hybridization analysis. One translocation, found in 6 different isolates, including the type strain, has a geographically widespread distribution and a unique haplotype and may be a marker of an incipient speciation event. All of the other translocations are unique. Exploitation of this karyotypic diversity may cast new light on both the biology of telomeres and centromeres and on isolating mechanisms in single-celled eukaryotes.",

author = "Brown, {William R A} and Gianni Liti and Carlos Rosa and Steve James and Ian Roberts and Vincent Robert and Neil Jolly and Wen Tang and Peter Baumann and Carter Green and Kristina Schlegel and Jonathan Young and Fabienne Hirchaud and Spencer Leek and Geraint Thomas and Anders Blomberg and Jonas Warringer",

year = "2011",

month = dec,

doi = "10.1534/g3.111.001123",

language = "English",

volume = "1",

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journal = "G3: Genes, Genomes, Genetics",

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Brown, WRA, Liti, G, Rosa, C, James, S, Roberts, I, Robert, V, Jolly, N, Tang, W, Baumann, P, Green, C, Schlegel, K, Young, J, Hirchaud, F, Leek, S, Thomas, G, Blomberg, A & Warringer, J 2011, 'A Geographically Diverse Collection of Schizosaccharomyces pombe Isolates Shows Limited Phenotypic Variation but Extensive Karyotypic Diversity', G3: Genes, Genomes, Genetics, vol. 1, no. 7, pp. 615-26. https://doi.org/10.1534/g3.111.001123

A Geographically Diverse Collection of Schizosaccharomyces pombe Isolates Shows Limited Phenotypic Variation but Extensive Karyotypic Diversity. / Brown, William R A; Liti, Gianni; Rosa, Carlos; James, Steve; Roberts, Ian; Robert, Vincent; Jolly, Neil; Tang, Wen; Baumann, Peter; Green, Carter; Schlegel, Kristina; Young, Jonathan; Hirchaud, Fabienne; Leek, Spencer; Thomas, Geraint; Blomberg, Anders; Warringer, Jonas.

In: G3: Genes, Genomes, Genetics, Vol. 1, No. 7, 12.2011, p. 615-26.

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

TY - JOUR

T1 - A Geographically Diverse Collection of Schizosaccharomyces pombe Isolates Shows Limited Phenotypic Variation but Extensive Karyotypic Diversity

AU - Brown, William R A

AU - Liti, Gianni

AU - Rosa, Carlos

AU - James, Steve

AU - Roberts, Ian

AU - Robert, Vincent

AU - Jolly, Neil

AU - Tang, Wen

AU - Baumann, Peter

AU - Green, Carter

AU - Schlegel, Kristina

AU - Young, Jonathan

AU - Hirchaud, Fabienne

AU - Leek, Spencer

AU - Thomas, Geraint

AU - Blomberg, Anders

AU - Warringer, Jonas

PY - 2011/12

Y1 - 2011/12

N2 - The fission yeast Schizosaccharomyces pombe has been widely used to study eukaryotic cell biology, but almost all of this work has used derivatives of a single strain. We have studied 81 independent natural isolates and 3 designated laboratory strains of Schizosaccharomyces pombe. Schizosaccharomyces pombe varies significantly in size but shows only limited variation in proliferation in different environments compared with Saccharomyces cerevisiae. Nucleotide diversity, π, at a near neutral site, the central core of the centromere of chromosome II is approximately 0.7%. Approximately 20% of the isolates showed karyotypic rearrangements as detected by pulsed field gel electrophoresis and filter hybridization analysis. One translocation, found in 6 different isolates, including the type strain, has a geographically widespread distribution and a unique haplotype and may be a marker of an incipient speciation event. All of the other translocations are unique. Exploitation of this karyotypic diversity may cast new light on both the biology of telomeres and centromeres and on isolating mechanisms in single-celled eukaryotes.

AB - The fission yeast Schizosaccharomyces pombe has been widely used to study eukaryotic cell biology, but almost all of this work has used derivatives of a single strain. We have studied 81 independent natural isolates and 3 designated laboratory strains of Schizosaccharomyces pombe. Schizosaccharomyces pombe varies significantly in size but shows only limited variation in proliferation in different environments compared with Saccharomyces cerevisiae. Nucleotide diversity, π, at a near neutral site, the central core of the centromere of chromosome II is approximately 0.7%. Approximately 20% of the isolates showed karyotypic rearrangements as detected by pulsed field gel electrophoresis and filter hybridization analysis. One translocation, found in 6 different isolates, including the type strain, has a geographically widespread distribution and a unique haplotype and may be a marker of an incipient speciation event. All of the other translocations are unique. Exploitation of this karyotypic diversity may cast new light on both the biology of telomeres and centromeres and on isolating mechanisms in single-celled eukaryotes.

U2 - 10.1534/g3.111.001123

DO - 10.1534/g3.111.001123

M3 - Article

C2 - 22384373

VL - 1

SP - 615

EP - 626

JO - G3: Genes, Genomes, Genetics

JF - G3: Genes, Genomes, Genetics

SN - 2160-1836

IS - 7

ER -