A high performance liquid chromatography tandem mass spectrometry protocol for detection of neurotransmitters in the rat brain tissue

Di Dai; Qian Qin; Xinyu Zhu; Qiuyuan Fang; Xianzong Meng; Lin Bai; Guang Yang; Ling Shan; Chunqing Liu

doi:10.1016/j.mex.2023.102083

A high performance liquid chromatography tandem mass spectrometry protocol for detection of neurotransmitters in the rat brain tissue

Di Dai, Qian Qin, Xinyu Zhu, Qiuyuan Fang, Xianzong Meng, Lin Bai, Guang Yang, Ling Shan, Chunqing Liu

Netherlands Institute for Neuroscience (NIN)

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

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Abstract

The detection of neurotransmitters has extensively been applied to the study of the pathogenesis, diagnosis, and therapeutic effect of drugs on many neuropsychiatric diseases. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) has been employed to determine neurotransmitters levels due to its distinct advantages. However, neurotransmitter detection still presents some challenges. A rapid and sensitive HPLC-MS/MS protocol has been established in our lab, which can simultaneously detect 5 neurotransmitters with an easy pretreatment procedure. The protocol provides demanded reference value for the lab using an Agilent HPLC-MS/MS system with a triple quadrupole analyzer.

Original language	English
Pages (from-to)	102083
Journal	MethodsX
Volume	10
DOIs	https://doi.org/10.1016/j.mex.2023.102083
Publication status	Published - 2023

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title = "A high performance liquid chromatography tandem mass spectrometry protocol for detection of neurotransmitters in the rat brain tissue",

abstract = "The detection of neurotransmitters has extensively been applied to the study of the pathogenesis, diagnosis, and therapeutic effect of drugs on many neuropsychiatric diseases. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) has been employed to determine neurotransmitters levels due to its distinct advantages. However, neurotransmitter detection still presents some challenges. A rapid and sensitive HPLC-MS/MS protocol has been established in our lab, which can simultaneously detect 5 neurotransmitters with an easy pretreatment procedure. The protocol provides demanded reference value for the lab using an Agilent HPLC-MS/MS system with a triple quadrupole analyzer.",

author = "Di Dai and Qian Qin and Xinyu Zhu and Qiuyuan Fang and Xianzong Meng and Lin Bai and Guang Yang and Ling Shan and Chunqing Liu",

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T1 - A high performance liquid chromatography tandem mass spectrometry protocol for detection of neurotransmitters in the rat brain tissue

AU - Dai, Di

AU - Qin, Qian

AU - Zhu, Xinyu

AU - Fang, Qiuyuan

AU - Meng, Xianzong

AU - Bai, Lin

AU - Yang, Guang

AU - Shan, Ling

AU - Liu, Chunqing

PY - 2023

Y1 - 2023

N2 - The detection of neurotransmitters has extensively been applied to the study of the pathogenesis, diagnosis, and therapeutic effect of drugs on many neuropsychiatric diseases. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) has been employed to determine neurotransmitters levels due to its distinct advantages. However, neurotransmitter detection still presents some challenges. A rapid and sensitive HPLC-MS/MS protocol has been established in our lab, which can simultaneously detect 5 neurotransmitters with an easy pretreatment procedure. The protocol provides demanded reference value for the lab using an Agilent HPLC-MS/MS system with a triple quadrupole analyzer.

AB - The detection of neurotransmitters has extensively been applied to the study of the pathogenesis, diagnosis, and therapeutic effect of drugs on many neuropsychiatric diseases. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) has been employed to determine neurotransmitters levels due to its distinct advantages. However, neurotransmitter detection still presents some challenges. A rapid and sensitive HPLC-MS/MS protocol has been established in our lab, which can simultaneously detect 5 neurotransmitters with an easy pretreatment procedure. The protocol provides demanded reference value for the lab using an Agilent HPLC-MS/MS system with a triple quadrupole analyzer.

U2 - 10.1016/j.mex.2023.102083

DO - 10.1016/j.mex.2023.102083

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SN - 2215-0161

VL - 10

SP - 102083

JO - MethodsX

JF - MethodsX

ER -

A high performance liquid chromatography tandem mass spectrometry protocol for detection of neurotransmitters in the rat brain tissue

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