A Long Noncoding RNA on the Ribosome Is Required for Lifespan Extension

TY - JOUR

T1 - A Long Noncoding RNA on the Ribosome Is Required for Lifespan Extension

AU - Essers, Paul B

AU - Nonnekens, Julie

AU - Goos, Yvonne J

AU - Betist, Marco C

AU - Viester, Marjon D

AU - Mossink, Britt

AU - Lansu, Nico

AU - Korswagen, Hendrik C

AU - Jelier, Rob

AU - Brenkman, Arjan B

AU - MacInnes, Alyson W

N1 - Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

PY - 2015/1/13

Y1 - 2015/1/13

N2 - The biogenesis of ribosomes and their coordination of protein translation consume an enormous amount of cellular energy. As such, it has been established that the inhibition of either process can extend eukaryotic lifespan. Here, we used next-generation sequencing to compare ribosome-associated RNAs from normal strains of Caenorhabditis elegans to those carrying the life-extending daf-2 mutation. We found a long noncoding RNA (lncRNA), transcribed telomeric sequence 1 (tts-1), on ribosomes of the daf-2 mutant. Depleting tts-1 in daf-2 mutants increases ribosome levels and significantly shortens their extended lifespan. We find tts-1 is also required for the longer lifespan of the mitochondrial clk-1 mutants but not the feeding-defective eat-2 mutants. In line with this, the clk-1 mutants express more tts-1 and fewer ribosomes than the eat-2 mutants. Our results suggest that the expression of tts-1 functions in different longevity pathways to reduce ribosome levels in a way that promotes life extension.

AB - The biogenesis of ribosomes and their coordination of protein translation consume an enormous amount of cellular energy. As such, it has been established that the inhibition of either process can extend eukaryotic lifespan. Here, we used next-generation sequencing to compare ribosome-associated RNAs from normal strains of Caenorhabditis elegans to those carrying the life-extending daf-2 mutation. We found a long noncoding RNA (lncRNA), transcribed telomeric sequence 1 (tts-1), on ribosomes of the daf-2 mutant. Depleting tts-1 in daf-2 mutants increases ribosome levels and significantly shortens their extended lifespan. We find tts-1 is also required for the longer lifespan of the mitochondrial clk-1 mutants but not the feeding-defective eat-2 mutants. In line with this, the clk-1 mutants express more tts-1 and fewer ribosomes than the eat-2 mutants. Our results suggest that the expression of tts-1 functions in different longevity pathways to reduce ribosome levels in a way that promotes life extension.

U2 - 10.1016/j.celrep.2014.12.029

DO - 10.1016/j.celrep.2014.12.029

M3 - Article

C2 - 25600869

SN - 2211-1247

JO - Cell Reports

JF - Cell Reports

ER -