TY - JOUR
T1 - A model for trans-kingdom pathogenicity in fonsecaea agents of human chromoblastomycosis
AU - Fornari, Gheniffer
AU - Gomes, Renata Rodrigues
AU - Degenhardt-Goldbach, Juliana
AU - Do sSantos, Suelen Silvana
AU - De Almeida, Sandro Rogério
AU - Dos Santos, Germana Davila
AU - Muro, Marisol Dominguez
AU - Bona, Cleusa
AU - Scola, Rosana Herminia
AU - Trindade, Edvaldo S.
AU - Bini, Israel Henrique
AU - Ferreira-Maba, Lisandra Santos
AU - Kestring, Daiane Rigoni
AU - Do Nascimento, Mariana Machado Fidelis
AU - De Souza Lima, Bruna Jacomel Favoreto
AU - Voidaleski, Morgana F.
AU - Steinmacher, Douglas André
AU - Da SilvaSoley, Bruna
AU - Deng, Shuwen
AU - Bocca, Anamelia Lorenzetti
AU - Da Silva, Moises B.
AU - Salgado, Claudio G.
AU - Pedroso E Silva De Azevedo, Conceição Maria
AU - Vicente, Vania Aparecida
AU - De Hoog, Sybren
PY - 2018/10/9
Y1 - 2018/10/9
N2 - The fungal genus Fonsecaea comprises etiological agents of human chromoblastomycosis, a chronic implantation skin disease. The current hypothesis is that patients acquire the infection through an injury from plant material. The present study aimed to evaluate a model of infection in plant and animal hosts to understand the parameters of trans-kingdom pathogenicity. Clinical strains of causative agents of chromoblastomycosis (Fonsecaea pedrosoi and Fonsecaea monophora) were compared with a strain of Fonsecaea erecta isolated from a living plant. The clinical strains of F. monophora and F. pedrosoi remained concentrated near the epidermis, whereas F. erecta colonized deeper plant tissues, resembling an endophytic behavior. In an invertebrate infection model with larvae of a beetle, Tenebrio molitor, F. erecta exhibited the lowest survival rates. However, F. pedrosoi produced dark, spherical to ovoidal cells that resembled muriform cells, the invasive form of human chromoblastomycosis confirming the role of muriform cells as a pathogenic adaptation in animal tissues. An immunologic assay in BALB/c mice demonstrated the high virulence of saprobic species in animal models was subsequently controlled via host higher immune response.
AB - The fungal genus Fonsecaea comprises etiological agents of human chromoblastomycosis, a chronic implantation skin disease. The current hypothesis is that patients acquire the infection through an injury from plant material. The present study aimed to evaluate a model of infection in plant and animal hosts to understand the parameters of trans-kingdom pathogenicity. Clinical strains of causative agents of chromoblastomycosis (Fonsecaea pedrosoi and Fonsecaea monophora) were compared with a strain of Fonsecaea erecta isolated from a living plant. The clinical strains of F. monophora and F. pedrosoi remained concentrated near the epidermis, whereas F. erecta colonized deeper plant tissues, resembling an endophytic behavior. In an invertebrate infection model with larvae of a beetle, Tenebrio molitor, F. erecta exhibited the lowest survival rates. However, F. pedrosoi produced dark, spherical to ovoidal cells that resembled muriform cells, the invasive form of human chromoblastomycosis confirming the role of muriform cells as a pathogenic adaptation in animal tissues. An immunologic assay in BALB/c mice demonstrated the high virulence of saprobic species in animal models was subsequently controlled via host higher immune response.
KW - Animal model
KW - Bactris gasipaes
KW - Chromoblastomycosis
KW - Fonsecaea
KW - Mimosa pudica
KW - Plant model
KW - Tenebrio molitor
KW - Virulence
U2 - 10.3389/fmicb.2018.02211
DO - 10.3389/fmicb.2018.02211
M3 - Article
SN - 1664-302X
VL - 9
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
M1 - 2211
ER -