A pendulum of induction between the epiblast and extra-embryonic endoderm supports post-implantation progression

Erik J Vrij, Yvonne S Scholte Op Reimer, Laury Roa Fuentes, Isabel Misteli Guerreiro, Viktoria Holzmann, Javier Frias Aldeguer, Giovanni Sestini, Bon-Kyoung Koo, Jop Kind, Clemens A van Blitterswijk, Nicolas C Rivron

Research output: Contribution to journal/periodicalArticleScientificpeer-review

6 Citations (Scopus)

Abstract

Embryogenesis is supported by dynamic loops of cellular interactions. Here, we create a partial mouse embryo model to elucidate the principles of epiblast (Epi) and extra-embryonic endoderm co-development (XEn). We trigger naive mouse embryonic stem cells to form a blastocyst-stage niche of Epi-like cells and XEn-like cells (3D, hydrogel free and serum free). Once established, these two lineages autonomously progress in minimal medium to form an inner pro-amniotic-like cavity surrounded by polarized Epi-like cells covered with visceral endoderm (VE)-like cells. The progression occurs through reciprocal inductions by which the Epi supports the primitive endoderm (PrE) to produce a basal lamina that subsequently regulates Epi polarization and/or cavitation, which, in return, channels the transcriptomic progression to VE. This VE then contributes to Epi bifurcation into anterior- and posterior-like states. Similarly, boosting the formation of PrE-like cells within blastoids supports developmental progression. We argue that self-organization can arise from lineage bifurcation followed by a pendulum of induction that propagates over time.

Original languageEnglish
JournalDevelopment (Cambridge)
Volume149
Issue number20
DOIs
Publication statusPublished - 15 Oct 2022

Keywords

  • Animals
  • Blastocyst
  • Cell Differentiation
  • Cell Lineage/physiology
  • Embryo Implantation
  • Embryo, Mammalian
  • Endoderm
  • Germ Layers
  • Mice

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