A simple remedy against artifactual double bands in denaturing gradient gel electrophoresis

I. Janse, J.M. Bok, G. Zwart

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    Abstract

    Denaturant gradient gel electrophoresis (DGGE) is a widely used method for mutation analysis and for studies of microbial diversity. Particular combinations of target gene fragments and primers may give rise to erroneous DGGE profiles. We report on a very straightforward means to eliminate the artifactual ‘double bands’ that can be encountered in several applications. [KEYWORDS: DGGE ; Double bands ; Artifacts]
    Original languageEnglish
    Pages (from-to)279-281
    JournalJournal of Microbiological Methods
    Volume57
    Issue number2
    DOIs
    Publication statusPublished - 2004

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