A survey of 16S rRNA and amoA genes related to autotrophic ammonia-oxidizing bacteria of the ß-subdivision of the class proteobacteria in contaminated groundwater

TY - JOUR

T1 - A survey of 16S rRNA and amoA genes related to autotrophic ammonia-oxidizing bacteria of the ß-subdivision of the class proteobacteria in contaminated groundwater

AU - Ivanova, I.A.

AU - Stephen, J.R.

AU - Chang, Y.J.

AU - Bruggemann, J.

AU - Long, P.E.

AU - McKinley, J.P.

AU - Kowalchuk, G.A.

AU - White, D.C.

AU - MacNaughton, S.J.

N1 - Reporting year: 2000 Metis note: 2749; CTE; TME ; ME file:///L:/Endnotedatabases/NIOOPUB/pdfs/Pdfs2000/Ivanova_ea_2749.pdf

PY - 2000

Y1 - 2000

N2 - In this study, we investigated the size and structure of autotrophic ammonia oxidizer (AAO) communities in the groundwater of a contamination plume originating from a mill- tailings disposal site. The site has high levels of dissolved N from anthropogenic sources, and exhibited wide variations in the concentrations of NO3- and NH3 + NH4+. Community structures were examined by PCR-DGGE targeting 16S rDNA with band excision and sequence analysis, and by analysis of amoA fragment clone libraries. AAO population sizes were estimated by competitive PCR targeting the gene amoA, and correlated significantly with nitrate concentration. Most samples revealed novel diversity in AAO 16S rDNA and amoA gene sequences. Both 16S rDNA and amoA analyses suggested that all samples were dominated by Nitrosomonas sp., Nitrosospira sp. being detected in only 3 of 15 samples. This study indicated numerical dominance of Nitrosomonas over Nitrosospira in groundwater, and suggests that groundwater ammonia oxidizers are more similar to those dominating freshwater sediments than bulk soil. [KEYWORDS: Nitrosomonas; Nitrosospira; nitrification; groundwater; amoA;16S Gradient gel-electrophoresis; ribosomal-rna genes; nitrosospira sp; nitrosomonas-europaea; molecular analysis; monooxygenase; sequences; soil; populations; diversity]

AB - In this study, we investigated the size and structure of autotrophic ammonia oxidizer (AAO) communities in the groundwater of a contamination plume originating from a mill- tailings disposal site. The site has high levels of dissolved N from anthropogenic sources, and exhibited wide variations in the concentrations of NO3- and NH3 + NH4+. Community structures were examined by PCR-DGGE targeting 16S rDNA with band excision and sequence analysis, and by analysis of amoA fragment clone libraries. AAO population sizes were estimated by competitive PCR targeting the gene amoA, and correlated significantly with nitrate concentration. Most samples revealed novel diversity in AAO 16S rDNA and amoA gene sequences. Both 16S rDNA and amoA analyses suggested that all samples were dominated by Nitrosomonas sp., Nitrosospira sp. being detected in only 3 of 15 samples. This study indicated numerical dominance of Nitrosomonas over Nitrosospira in groundwater, and suggests that groundwater ammonia oxidizers are more similar to those dominating freshwater sediments than bulk soil. [KEYWORDS: Nitrosomonas; Nitrosospira; nitrification; groundwater; amoA;16S Gradient gel-electrophoresis; ribosomal-rna genes; nitrosospira sp; nitrosomonas-europaea; molecular analysis; monooxygenase; sequences; soil; populations; diversity]

U2 - 10.1139/cjm-46-11-1012

DO - 10.1139/cjm-46-11-1012

M3 - Article

SN - 0008-4166

VL - 46

SP - 1012

EP - 1020

JO - Canadian Journal of Microbiology

JF - Canadian Journal of Microbiology

IS - 11

ER -