AAV-CRB2 protects against vision loss in an inducible CRB1 retinitis pigmentosa mouse model

Thilo M. Buck, Rogier M. Vos, C. Henrique Alves, Jan Wijnholds

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

Loss of Crumbs homolog 1 (CRB1) or CRB2 proteins in Müller cells or photoreceptors in the mouse retina results in a CRB dose-dependent retinal phenotype. In this study, we present a novel Müller cell-specific Crb1KOCrb2LowMGC retinitis pigmentosa mouse model (complete loss of CRB1 and reduced levels of CRB2 specifically in Müller cells). The Crb double mutant mice showed deficits in electroretinography, optokinetic head tracking, and retinal morphology. Exposure of retinas to low levels of DL-α-aminoadipate acid induced gliosis and retinal disorganization in Crb1KOCrb2LowMGC retinas but not in wild-type or Crb1-deficient retinas. Crb1KOCrb2LowMGC mice showed a substantial decrease in inner/outer photoreceptor segment length and optokinetic head-tracking response. Intravitreal application of rAAV vectors expressing human CRB2 (hCRB2) in Müller cells of Crb1KOCrb2LowMGC mice subsequently exposed to low levels of DL-α-aminoadipate acid prevented loss of vision, whereas recombinant adeno-associated viral (rAAV) vectors expressing human CRB1 (hCRB1) did not. Both rAAV vectors partially protected the morphology of the retina. The results suggest that hCRB expression in Müller cells is vital for control of retinal cell adhesion at the outer limiting membrane, and that the rAAV-cytomegalovirus (CMV)-hCRB2 vector is more potent than rAAV-minimal CMV (CMVmin)-hCRB1 in protection against loss of vision.

Original languageEnglish
Pages (from-to)423-441
Number of pages19
JournalMolecular Therapy - Methods and Clinical Development
Volume20
DOIs
Publication statusPublished - 2021

Keywords

  • AAV
  • adeno-associated viral vector
  • CRB1
  • CRB2
  • DL-AAA
  • Müller glial cell
  • retinitis pigmentosa

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