Accumulation of recalcitrant xylan in mushroom-compost is due to a lack of xylan substituent removing enzyme activities of Agaricus bisporus

Edita Jurak; Aleksandrina Patyshakuliyeva; Dimitris Kapsokalyvas; Lia Xing; Marc A M J van Zandvoort; Ronald P de Vries; Harry Gruppen; Mirjam A Kabel

doi:10.1016/j.carbpol.2015.06.065

Accumulation of recalcitrant xylan in mushroom-compost is due to a lack of xylan substituent removing enzyme activities of Agaricus bisporus

Edita Jurak, Aleksandrina Patyshakuliyeva, Dimitris Kapsokalyvas, Lia Xing, Marc A M J van Zandvoort, Ronald P de Vries, Harry Gruppen, Mirjam A Kabel

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

7 Citations (Scopus)

Abstract

The ability of Agaricus bisporus to degrade xylan in wheat straw based compost during mushroom formation is unclear. In this paper, xylan was extracted from the compost with water, 1M and 4M alkali. Over the phases analyzed, the remaining xylan was increasingly substituted with (4-O-methyl-)glucuronic acid and arabinosyl residues, both one and two arabinosyl residues per xylosyl residue remained. In the 1M and 4M KOH soluble solids of spent compost, 33 and 49 out of 100 xylosyl residues, respectively, were substituted. The accumulation of glucuronic acid substituents matched with the analysis that the two A. bisporus genes encoding for α-glucuronidase activity (both GH115) were not expressed in the A. bisporus mycelium in the compost during fruiting. Also, in a maximum likelihood tree it was shown that it is not likely that A. bisporus possesses genes encoding for the activity to remove arabinose from xylosyl residues having two arabinosyl residues.

Original language	English
Pages (from-to)	359-68
Number of pages	10
Journal	Carbohydrate Polymers
Volume	132
DOIs	https://doi.org/10.1016/j.carbpol.2015.06.065
Publication status	Published - 05 Nov 2015

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10.1016/j.carbpol.2015.06.065

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@article{ceafb42b2b2e489ebf0891ba7f2200b2,

title = "Accumulation of recalcitrant xylan in mushroom-compost is due to a lack of xylan substituent removing enzyme activities of Agaricus bisporus",

abstract = "The ability of Agaricus bisporus to degrade xylan in wheat straw based compost during mushroom formation is unclear. In this paper, xylan was extracted from the compost with water, 1M and 4M alkali. Over the phases analyzed, the remaining xylan was increasingly substituted with (4-O-methyl-)glucuronic acid and arabinosyl residues, both one and two arabinosyl residues per xylosyl residue remained. In the 1M and 4M KOH soluble solids of spent compost, 33 and 49 out of 100 xylosyl residues, respectively, were substituted. The accumulation of glucuronic acid substituents matched with the analysis that the two A. bisporus genes encoding for α-glucuronidase activity (both GH115) were not expressed in the A. bisporus mycelium in the compost during fruiting. Also, in a maximum likelihood tree it was shown that it is not likely that A. bisporus possesses genes encoding for the activity to remove arabinose from xylosyl residues having two arabinosyl residues.",

author = "Edita Jurak and Aleksandrina Patyshakuliyeva and Dimitris Kapsokalyvas and Lia Xing and {van Zandvoort}, {Marc A M J} and {de Vries}, {Ronald P} and Harry Gruppen and Kabel, {Mirjam A}",

year = "2015",

month = nov,

day = "5",

doi = "10.1016/j.carbpol.2015.06.065",

language = "English",

volume = "132",

pages = "359--68",

journal = "Carbohydrate Polymers",

issn = "0144-8617",

publisher = "Elsevier B.V.",

}

Accumulation of recalcitrant xylan in mushroom-compost is due to a lack of xylan substituent removing enzyme activities of Agaricus bisporus. / Jurak, Edita; Patyshakuliyeva, Aleksandrina; Kapsokalyvas, Dimitris et al.
In: Carbohydrate Polymers, Vol. 132, 05.11.2015, p. 359-68.

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

TY - JOUR

T1 - Accumulation of recalcitrant xylan in mushroom-compost is due to a lack of xylan substituent removing enzyme activities of Agaricus bisporus

AU - Jurak, Edita

AU - Patyshakuliyeva, Aleksandrina

AU - Kapsokalyvas, Dimitris

AU - Xing, Lia

AU - van Zandvoort, Marc A M J

AU - de Vries, Ronald P

AU - Gruppen, Harry

AU - Kabel, Mirjam A

PY - 2015/11/5

Y1 - 2015/11/5

N2 - The ability of Agaricus bisporus to degrade xylan in wheat straw based compost during mushroom formation is unclear. In this paper, xylan was extracted from the compost with water, 1M and 4M alkali. Over the phases analyzed, the remaining xylan was increasingly substituted with (4-O-methyl-)glucuronic acid and arabinosyl residues, both one and two arabinosyl residues per xylosyl residue remained. In the 1M and 4M KOH soluble solids of spent compost, 33 and 49 out of 100 xylosyl residues, respectively, were substituted. The accumulation of glucuronic acid substituents matched with the analysis that the two A. bisporus genes encoding for α-glucuronidase activity (both GH115) were not expressed in the A. bisporus mycelium in the compost during fruiting. Also, in a maximum likelihood tree it was shown that it is not likely that A. bisporus possesses genes encoding for the activity to remove arabinose from xylosyl residues having two arabinosyl residues.

AB - The ability of Agaricus bisporus to degrade xylan in wheat straw based compost during mushroom formation is unclear. In this paper, xylan was extracted from the compost with water, 1M and 4M alkali. Over the phases analyzed, the remaining xylan was increasingly substituted with (4-O-methyl-)glucuronic acid and arabinosyl residues, both one and two arabinosyl residues per xylosyl residue remained. In the 1M and 4M KOH soluble solids of spent compost, 33 and 49 out of 100 xylosyl residues, respectively, were substituted. The accumulation of glucuronic acid substituents matched with the analysis that the two A. bisporus genes encoding for α-glucuronidase activity (both GH115) were not expressed in the A. bisporus mycelium in the compost during fruiting. Also, in a maximum likelihood tree it was shown that it is not likely that A. bisporus possesses genes encoding for the activity to remove arabinose from xylosyl residues having two arabinosyl residues.

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DO - 10.1016/j.carbpol.2015.06.065

M3 - Article

C2 - 26256360

SN - 0144-8617

VL - 132

SP - 359

EP - 368

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

ER -

Accumulation of recalcitrant xylan in mushroom-compost is due to a lack of xylan substituent removing enzyme activities of Agaricus bisporus

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