Adenoviral vector-mediated expression of B-50/GAP-43 induces alterations in the membrane organization of olfactory axon terminals in vivo

Anthony J D G Holtmaat, W.T.J.M.C. Hermens, M.A.F. Sonnemans, Roman J Giger, F W Van Leeuwen, M G Kaplitt, A B Oestreicher, Willem Hendrik Gispen, J Verhaagen

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

B-50/GAP-43 is an intraneuronal membrane-associated growth cone protein with an important role in axonal growth and regeneration. By using adenoviral vector-directed expression of B-50/GAP-43 we studied the morphogenic action of B-50/GAP-43 in mature primary olfactory neurons that have established functional synaptic connections. B-50/GAP-43 induced gradual alterations in the morphology of olfactory synapses. In the first days after overexpression, small protrusions originating from the preterminal axon shaft and from the actual synaptic bouton were formed. With time the progressive formation of multiple ultraterminal branches resulted in axonal labyrinths composed of tightly packed sheaths of neuronal membrane. Thus, B-50/GAP-43 is a protein that can promote neuronal membrane expansion at synaptic boutons. This function of B-50/GAP-43 suggests that this protein may subserve an important role in ongoing structural synaptic plasticity in adult neurons and in neuronal membrane repair after injury to synaptic fields.

Original languageEnglish
Pages (from-to)6575-86
Number of pages12
JournalThe Journal of neuroscience : the official journal of the Society for Neuroscience
Volume17
Issue number17
Publication statusPublished - 01 Sep 1997

Keywords

  • Adenoviridae
  • Animals
  • Axons
  • Cercopithecus aethiops
  • Epithelium
  • GAP-43 Protein
  • Genetic Vectors
  • Membrane Glycoproteins
  • Nerve Endings
  • Nerve Tissue Proteins
  • Neuronal Plasticity
  • Olfactory Pathways
  • Synapses
  • Synaptic Transmission
  • Vero Cells
  • Journal Article
  • Research Support, Non-U.S. Gov't

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  • Cite this

    Holtmaat, A. J. D. G., Hermens, W. T. J. M. C., Sonnemans, M. A. F., Giger, R. J., Van Leeuwen, F. W., Kaplitt, M. G., Oestreicher, A. B., Gispen, W. H., & Verhaagen, J. (1997). Adenoviral vector-mediated expression of B-50/GAP-43 induces alterations in the membrane organization of olfactory axon terminals in vivo. The Journal of neuroscience : the official journal of the Society for Neuroscience, 17(17), 6575-86.