Agar composition affects in vitro screening of biocontrol activity of antagonistic microorganisms

Lien Bosmans; I. De Bruijn; Rene de Mot; Hans Readers; Bart Lievens

doi:10.1016/j.mimet.2016.05.004

Agar composition affects in vitro screening of biocontrol activity of antagonistic microorganisms

Lien Bosmans, I. De Bruijn, Rene de Mot, Hans Readers, Bart Lievens

NIOO - Microbial Ecology (ME)

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Abstract

Agar-based screening assays are the method of choice when evaluating antagonistic potential of bacterial
biocontrol-candidates against pathogens.Weshowed thatwhen using the samemedium, but different agar compositions,
the activity of a bacterial antagonist against Agrobacteriumwas strongly affected. Consequently, results
from in vitro screenings should be interpreted cautiously.

Original language	English
Pages (from-to)	7-9
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	127
DOIs	https://doi.org/10.1016/j.mimet.2016.05.004
Publication status	Published - 07 May 2016

Keywords

Agar composition
Agar in vitro screening
Biocontrol activity
international

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10.1016/j.mimet.2016.05.004

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title = "Agar composition affects in vitro screening of biocontrol activity of antagonistic microorganisms",

abstract = "Agar-based screening assays are the method of choice when evaluating antagonistic potential of bacterialbiocontrol-candidates against pathogens.Weshowed thatwhen using the samemedium, but different agar compositions,the activity of a bacterial antagonist against Agrobacteriumwas strongly affected. Consequently, resultsfrom in vitro screenings should be interpreted cautiously.",

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author = "Lien Bosmans and {De Bruijn}, I. and {de Mot}, Rene and Hans Readers and Bart Lievens",

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AU - Bosmans, Lien

AU - De Bruijn, I.

AU - de Mot, Rene

AU - Readers, Hans

AU - Lievens, Bart

N1 - 6120, ME; Data archiving: Property of and archived at University Leuven

PY - 2016/5/7

Y1 - 2016/5/7

N2 - Agar-based screening assays are the method of choice when evaluating antagonistic potential of bacterialbiocontrol-candidates against pathogens.Weshowed thatwhen using the samemedium, but different agar compositions,the activity of a bacterial antagonist against Agrobacteriumwas strongly affected. Consequently, resultsfrom in vitro screenings should be interpreted cautiously.

AB - Agar-based screening assays are the method of choice when evaluating antagonistic potential of bacterialbiocontrol-candidates against pathogens.Weshowed thatwhen using the samemedium, but different agar compositions,the activity of a bacterial antagonist against Agrobacteriumwas strongly affected. Consequently, resultsfrom in vitro screenings should be interpreted cautiously.

KW - Agar composition

KW - Agar in vitro screening

KW - Biocontrol activity

KW - international

U2 - 10.1016/j.mimet.2016.05.004

DO - 10.1016/j.mimet.2016.05.004

M3 - Article

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SP - 7

EP - 9

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

ER -

Agar composition affects in vitro screening of biocontrol activity of antagonistic microorganisms

Abstract

Keywords

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