Allele-specific detection of single mRNA molecules in situ

C.H. Hansen, A. van Oudenaarden

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

We describe a method for fluorescence in situ identification of individual mRNA molecules, allowing quantitative and accurate measurement, in single cells, of allele-specific transcripts that differ by only a few nucleotides. By using a combination of allele-specific and non-allele-specific probe libraries, we achieve >95% detection accuracy. We investigate the allele-specific stochastic expression of Nanog, which encodes a pluripotency factor, in murine embryonic stem cells.
Original languageEnglish
Pages (from-to)869-871
JournalNature Methods
Volume10
Issue number9
DOIs
Publication statusPublished - 2013

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