Allele-specific detection of single mRNA molecules in situ

TY - JOUR

T1 - Allele-specific detection of single mRNA molecules in situ

AU - Hansen, C.H.

AU - van Oudenaarden, A.

N1 - Reporting year: 2013

PY - 2013

Y1 - 2013

N2 - We describe a method for fluorescence in situ identification of individual mRNA molecules, allowing quantitative and accurate measurement, in single cells, of allele-specific transcripts that differ by only a few nucleotides. By using a combination of allele-specific and non-allele-specific probe libraries, we achieve >95% detection accuracy. We investigate the allele-specific stochastic expression of Nanog, which encodes a pluripotency factor, in murine embryonic stem cells.

AB - We describe a method for fluorescence in situ identification of individual mRNA molecules, allowing quantitative and accurate measurement, in single cells, of allele-specific transcripts that differ by only a few nucleotides. By using a combination of allele-specific and non-allele-specific probe libraries, we achieve >95% detection accuracy. We investigate the allele-specific stochastic expression of Nanog, which encodes a pluripotency factor, in murine embryonic stem cells.

U2 - 10.1038/NMETH.2601

DO - 10.1038/NMETH.2601

M3 - Article

SN - 1548-7091

VL - 10

SP - 869

EP - 871

JO - Nature Methods

JF - Nature Methods

IS - 9

ER -