An automated multiplexed turbidometric and data collection system for measuring growth kinetics of anaerobes dependent on gaseous substrates

TY - JOUR

T1 - An automated multiplexed turbidometric and data collection system for measuring growth kinetics of anaerobes dependent on gaseous substrates

AU - Hunt, Kristopher A.

AU - Forbes, Jonathan

AU - Taub, Fred

AU - Elliott, Nicholas

AU - Hardwicke, Jessica

AU - Petersen, Robert

AU - Stopnisek, Nejc

AU - Beck, David A.C.

AU - Stahl, David A.

N1 - 7278, ME; Data archiving: data archived at publisher (not NIOO data)

PY - 2021/9

Y1 - 2021/9

N2 - Standard methods of monitoring the growth kinetics of anaerobic microorganisms are generally impractical when there is a protracted or indeterminate period of active growth, and when high numbers of samples or replications are required. As part of our studies of the adaptive evolution of a simple anaerobic syntrophic mutualism, requiring the characterization of many isolates and alternative syntrophic pairings, we developed a multiplexed growth monitoring system using a combination of commercially available electronics and custom designed circuitry and materials. This system automatically monitors up to 64 sealed, and as needed pressurized, culture tubes and reports the growth data in real-time through integration with a customized relational database. The utility of this system was demonstrated by resolving minor differences in growth kinetics associated with the adaptive evolution of a simple microbial community comprised of a sulfate reducing bacterium, Desulfovibrio vulgaris, grown in syntrophic association with Methanococcus maripaludis, a hydrogenotrophic methanogen.

AB - Standard methods of monitoring the growth kinetics of anaerobic microorganisms are generally impractical when there is a protracted or indeterminate period of active growth, and when high numbers of samples or replications are required. As part of our studies of the adaptive evolution of a simple anaerobic syntrophic mutualism, requiring the characterization of many isolates and alternative syntrophic pairings, we developed a multiplexed growth monitoring system using a combination of commercially available electronics and custom designed circuitry and materials. This system automatically monitors up to 64 sealed, and as needed pressurized, culture tubes and reports the growth data in real-time through integration with a customized relational database. The utility of this system was demonstrated by resolving minor differences in growth kinetics associated with the adaptive evolution of a simple microbial community comprised of a sulfate reducing bacterium, Desulfovibrio vulgaris, grown in syntrophic association with Methanococcus maripaludis, a hydrogenotrophic methanogen.

KW - Anaerobic

KW - Automated

KW - Microbial

KW - Multiplex

KW - Optical density

KW - international

KW - Plan_S-Compliant_NO

U2 - 10.1016/j.mimet.2021.106294

DO - 10.1016/j.mimet.2021.106294

M3 - Article

AN - SCOPUS:85111921631

SN - 0167-7012

VL - 188

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

M1 - 106294

ER -