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An ecogenomic analysis of herbivore-induced plant volatiles in Brassica juncea. / Mathur, V.; Tytgat, T.O.G.; Hordijk, C.A.; Harhangi, H.R.; Jansen, J.J.; Reddy, A.S.; Harvey, J.A.; Vet, L.E.M.; Van Dam, N.M.

In: Molecular Ecology, Vol. 22, No. 24, 2013, p. 6179-6196.

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@article{a5bf54e9e3a74585a2d0f4a7ca6b7635,
title = "An ecogenomic analysis of herbivore-induced plant volatiles in Brassica juncea",
abstract = "[KEYWORDS: gene expression green leaf volatiles mustard parasitoids Spodoptera sulphides] Upon herbivore feeding, plants emit complex bouquets of induced volatiles that may repel insect herbivores as well as attract parasitoids or predators. Due to differences in the temporal dynamics of individual components, the composition of the herbivore-induced plant volatile (HIPV) blend changes with time. Consequently, the response of insects associated with plants is not constant either. Using Brassica juncea as the model plant and generalist Spodoptera spp. larvae as the inducing herbivore, we investigated herbivore and parasitoid preference as well as the molecular mechanisms behind the temporal dynamics in HIPV emissions at 24, 48 and 72 h after damage. In choice tests, Spodoptera litura moth preferred undamaged plants, whereas its parasitoid Cotesia marginiventris favoured plants induced for 48 h. In contrast, the specialist Plutella xylostella and its parasitoid C. vestalis preferred plants induced for 72 h. These preferences matched the dynamic changes in HIPV blends over time. Gene expression analysis suggested that the induced response after Spodoptera feeding is mainly controlled by the jasmonic acid pathway in both damaged and systemic leaves. Several genes involved in sulphide and green leaf volatile synthesis were clearly up-regulated. This study thus shows that HIPV blends vary considerably over a short period of time, and these changes are actively regulated at the gene expression level. Moreover, temporal changes in HIPVs elicit differential preferences of herbivores and their natural enemies. We argue that the temporal dynamics of HIPVs may play a key role in shaping the response of insects associated with plants.",
keywords = "international",
author = "V. Mathur and T.O.G. Tytgat and C.A. Hordijk and H.R. Harhangi and J.J. Jansen and A.S. Reddy and J.A. Harvey and L.E.M. Vet and {Van Dam}, N.M.",
note = "Reporting year: 2013 Metis note: 5568; TE",
year = "2013",
doi = "10.1111/mec.12555",
language = "English",
volume = "22",
pages = "6179--6196",
journal = "Molecular Ecology",
issn = "0962-1083",
publisher = "Wiley-Blackwell",
number = "24",

}

RIS

TY - JOUR

T1 - An ecogenomic analysis of herbivore-induced plant volatiles in Brassica juncea

AU - Mathur, V.

AU - Tytgat, T.O.G.

AU - Hordijk, C.A.

AU - Harhangi, H.R.

AU - Jansen, J.J.

AU - Reddy, A.S.

AU - Harvey, J.A.

AU - Vet, L.E.M.

AU - Van Dam, N.M.

N1 - Reporting year: 2013 Metis note: 5568; TE

PY - 2013

Y1 - 2013

N2 - [KEYWORDS: gene expression green leaf volatiles mustard parasitoids Spodoptera sulphides] Upon herbivore feeding, plants emit complex bouquets of induced volatiles that may repel insect herbivores as well as attract parasitoids or predators. Due to differences in the temporal dynamics of individual components, the composition of the herbivore-induced plant volatile (HIPV) blend changes with time. Consequently, the response of insects associated with plants is not constant either. Using Brassica juncea as the model plant and generalist Spodoptera spp. larvae as the inducing herbivore, we investigated herbivore and parasitoid preference as well as the molecular mechanisms behind the temporal dynamics in HIPV emissions at 24, 48 and 72 h after damage. In choice tests, Spodoptera litura moth preferred undamaged plants, whereas its parasitoid Cotesia marginiventris favoured plants induced for 48 h. In contrast, the specialist Plutella xylostella and its parasitoid C. vestalis preferred plants induced for 72 h. These preferences matched the dynamic changes in HIPV blends over time. Gene expression analysis suggested that the induced response after Spodoptera feeding is mainly controlled by the jasmonic acid pathway in both damaged and systemic leaves. Several genes involved in sulphide and green leaf volatile synthesis were clearly up-regulated. This study thus shows that HIPV blends vary considerably over a short period of time, and these changes are actively regulated at the gene expression level. Moreover, temporal changes in HIPVs elicit differential preferences of herbivores and their natural enemies. We argue that the temporal dynamics of HIPVs may play a key role in shaping the response of insects associated with plants.

AB - [KEYWORDS: gene expression green leaf volatiles mustard parasitoids Spodoptera sulphides] Upon herbivore feeding, plants emit complex bouquets of induced volatiles that may repel insect herbivores as well as attract parasitoids or predators. Due to differences in the temporal dynamics of individual components, the composition of the herbivore-induced plant volatile (HIPV) blend changes with time. Consequently, the response of insects associated with plants is not constant either. Using Brassica juncea as the model plant and generalist Spodoptera spp. larvae as the inducing herbivore, we investigated herbivore and parasitoid preference as well as the molecular mechanisms behind the temporal dynamics in HIPV emissions at 24, 48 and 72 h after damage. In choice tests, Spodoptera litura moth preferred undamaged plants, whereas its parasitoid Cotesia marginiventris favoured plants induced for 48 h. In contrast, the specialist Plutella xylostella and its parasitoid C. vestalis preferred plants induced for 72 h. These preferences matched the dynamic changes in HIPV blends over time. Gene expression analysis suggested that the induced response after Spodoptera feeding is mainly controlled by the jasmonic acid pathway in both damaged and systemic leaves. Several genes involved in sulphide and green leaf volatile synthesis were clearly up-regulated. This study thus shows that HIPV blends vary considerably over a short period of time, and these changes are actively regulated at the gene expression level. Moreover, temporal changes in HIPVs elicit differential preferences of herbivores and their natural enemies. We argue that the temporal dynamics of HIPVs may play a key role in shaping the response of insects associated with plants.

KW - international

U2 - 10.1111/mec.12555

DO - 10.1111/mec.12555

M3 - Article

VL - 22

SP - 6179

EP - 6196

JO - Molecular Ecology

JF - Molecular Ecology

SN - 0962-1083

IS - 24

ER -

ID: 179627