Analysis of 16S rRNA and mxaF genes revealing insights into Methylobacterium niche-specific plant association

Manuella Nobrega Dourado; Fernando Dini Andreote; Francisco Dini-Andreote; Raphael Conti; Janete Magali Araujo; Welington Luiz Araujo

doi:10.1590/S1415-47572012005000017

Analysis of 16S rRNA and mxaF genes revealing insights into Methylobacterium niche-specific plant association

Manuella Nobrega Dourado, Fernando Dini Andreote, Francisco Dini-Andreote, Raphael Conti, Janete Magali Araujo, Welington Luiz Araujo

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

Abstract

The genus Methylobacterium comprises pink-pigmented facultative methylotrophic (PPFM) bacteria, known to be an important plant-associated bacterial group. Species of this group, described as plant-nodulating, have the dual capacity of producing cytokinin and enzymes, such as pectinase and cellulase, involved in systemic resistance induction and nitrogen fixation under specific plant environmental conditions. The aim hereby was to evaluate the phylogenetic distribution of Methylobacterium spp. isolates from different host plants. Thus, a comparative analysis between sequences from structural (16S rRNA) and functional mxaF (which codifies for a subunit of the enzyme methanol dehydrogenase) ubiquitous genes, was undertaken. Notably, some Methylobacterium spp. isolates are generalists through colonizing more than one host plant, whereas others are exclusively found in certain specific plant-species. Congruency between phylogeny and specific host inhabitance was higher in the mxaF gene than in the 16S rRNA, a possible indication of function-based selection in this niche. Therefore, in a first stage, plant colonization by Methylobacterium spp. could represent generalist behavior, possibly related to microbial competition and adaptation to a plant environment. Otherwise, niche-specific colonization is apparently impelled by the host plant.

Original language	English
Pages (from-to)	142-148
Journal	Genetics and Molecular Biology
Volume	35
Issue number	1
DOIs	https://doi.org/10.1590/S1415-47572012005000017 https://doi.org/10.1590/S1415-47572012005000017
Publication status	Published - 2012
Externally published	Yes

Keywords

phylogenetic diversity
methylotrofics
PPFM
plant-bacteria interaction

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title = "Analysis of 16S rRNA and mxaF genes revealing insights into Methylobacterium niche-specific plant association",

abstract = "The genus Methylobacterium comprises pink-pigmented facultative methylotrophic (PPFM) bacteria, known to be an important plant-associated bacterial group. Species of this group, described as plant-nodulating, have the dual capacity of producing cytokinin and enzymes, such as pectinase and cellulase, involved in systemic resistance induction and nitrogen fixation under specific plant environmental conditions. The aim hereby was to evaluate the phylogenetic distribution of Methylobacterium spp. isolates from different host plants. Thus, a comparative analysis between sequences from structural (16S rRNA) and functional mxaF (which codifies for a subunit of the enzyme methanol dehydrogenase) ubiquitous genes, was undertaken. Notably, some Methylobacterium spp. isolates are generalists through colonizing more than one host plant, whereas others are exclusively found in certain specific plant-species. Congruency between phylogeny and specific host inhabitance was higher in the mxaF gene than in the 16S rRNA, a possible indication of function-based selection in this niche. Therefore, in a first stage, plant colonization by Methylobacterium spp. could represent generalist behavior, possibly related to microbial competition and adaptation to a plant environment. Otherwise, niche-specific colonization is apparently impelled by the host plant.",

keywords = "phylogenetic diversity, methylotrofics, PPFM, plant-bacteria interaction",

author = "Dourado, {Manuella Nobrega} and Andreote, {Fernando Dini} and Francisco Dini-Andreote and Raphael Conti and Araujo, {Janete Magali} and Araujo, {Welington Luiz}",

year = "2012",

doi = "10.1590/S1415-47572012005000017",

language = "English",

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T1 - Analysis of 16S rRNA and mxaF genes revealing insights into Methylobacterium niche-specific plant association

AU - Dourado, Manuella Nobrega

AU - Andreote, Fernando Dini

AU - Dini-Andreote, Francisco

AU - Conti, Raphael

AU - Araujo, Janete Magali

AU - Araujo, Welington Luiz

PY - 2012

Y1 - 2012

N2 - The genus Methylobacterium comprises pink-pigmented facultative methylotrophic (PPFM) bacteria, known to be an important plant-associated bacterial group. Species of this group, described as plant-nodulating, have the dual capacity of producing cytokinin and enzymes, such as pectinase and cellulase, involved in systemic resistance induction and nitrogen fixation under specific plant environmental conditions. The aim hereby was to evaluate the phylogenetic distribution of Methylobacterium spp. isolates from different host plants. Thus, a comparative analysis between sequences from structural (16S rRNA) and functional mxaF (which codifies for a subunit of the enzyme methanol dehydrogenase) ubiquitous genes, was undertaken. Notably, some Methylobacterium spp. isolates are generalists through colonizing more than one host plant, whereas others are exclusively found in certain specific plant-species. Congruency between phylogeny and specific host inhabitance was higher in the mxaF gene than in the 16S rRNA, a possible indication of function-based selection in this niche. Therefore, in a first stage, plant colonization by Methylobacterium spp. could represent generalist behavior, possibly related to microbial competition and adaptation to a plant environment. Otherwise, niche-specific colonization is apparently impelled by the host plant.

AB - The genus Methylobacterium comprises pink-pigmented facultative methylotrophic (PPFM) bacteria, known to be an important plant-associated bacterial group. Species of this group, described as plant-nodulating, have the dual capacity of producing cytokinin and enzymes, such as pectinase and cellulase, involved in systemic resistance induction and nitrogen fixation under specific plant environmental conditions. The aim hereby was to evaluate the phylogenetic distribution of Methylobacterium spp. isolates from different host plants. Thus, a comparative analysis between sequences from structural (16S rRNA) and functional mxaF (which codifies for a subunit of the enzyme methanol dehydrogenase) ubiquitous genes, was undertaken. Notably, some Methylobacterium spp. isolates are generalists through colonizing more than one host plant, whereas others are exclusively found in certain specific plant-species. Congruency between phylogeny and specific host inhabitance was higher in the mxaF gene than in the 16S rRNA, a possible indication of function-based selection in this niche. Therefore, in a first stage, plant colonization by Methylobacterium spp. could represent generalist behavior, possibly related to microbial competition and adaptation to a plant environment. Otherwise, niche-specific colonization is apparently impelled by the host plant.

KW - phylogenetic diversity

KW - methylotrofics

KW - PPFM

KW - plant-bacteria interaction

U2 - 10.1590/S1415-47572012005000017

DO - 10.1590/S1415-47572012005000017

M3 - Article

SN - 1415-4757

VL - 35

SP - 142

EP - 148

JO - Genetics and Molecular Biology

JF - Genetics and Molecular Biology

IS - 1

ER -

Analysis of 16S rRNA and mxaF genes revealing insights into Methylobacterium niche-specific plant association

Abstract

Keywords

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