Analysis of the molecular basis for the non-amylolytic and non-proteolytic nature of Aspergillus vadensis CBS 113365

Dujuan Liu, Sandra Garrigues, Helena Culleton, Vincent A. McKie, Ronald P. de Vries*

*Corresponding author for this work

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

Aspergillus vadensis CBS 113365, a close relative of A. niger, has been suggested as a more favourable alternative for recombinant protein production as it does not acidify the culture medium and produces very low levels of extracellular proteases. The aim of this study was to investigate the underlying cause of the non-amylolytic and non-proteolytic phenotype of A. vadensis CBS 113365. Our results demonstrate that the non-functionality of the amylolytic transcription factor AmyR in A. vadensis CBS 113365 is primarily attributed to the lack of functionality of its gene's promoter sequence. In contrast, a different mechanism is likely causing the lack of PrtT activity, which is the main transcriptional regulator of protease production. The findings presented here not only expand our understanding of the genetic basis behind the distinct characteristics of A. vadensis CBS 113365, but also underscore its potential as a favourable alternative for recombinant protein production.

Original languageEnglish
Pages (from-to)25-32
Number of pages8
JournalNew Biotechnology
Volume82
DOIs
Publication statusPublished - 25 Sept 2024

Keywords

  • AmyR
  • Aspergillus vadensis
  • Gene regulation
  • Genetic swaps
  • PrtT
  • Transcriptional activator

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