Antifungal and antioxidant activities of the phytomedicine pipsissewa, Chimaphila umbellata.

I. Galván, N. Mir-Rashed, M. Jessulat, M. Atanya, A. Golshani, T. Boekhout, T. Durst, P. Petit, V.T. Amiguet, R.C. Summerbell, I. Cruz, J.T. Arnason, M.L. Smith

    Research output: Contribution to journal/periodicalArticleScientificpeer-review

    40 Citations (Scopus)

    Abstract

    Bioassay-guided fractionation of Chimaphila umbellata (L.) W. Bart (Pyrolaceae) ethanol extracts led to the identification of 2,7-dimethyl-1,4-naphthoquinone (chimaphilin) as the principal antifungal component. The structure of chimaphilin was confirmed by 1H and 13C NMR spectroscopy. The antifungal activity of chimaphilin was evaluated using the microdilution method with Saccharomyces cerevisiae (0.05mg/mL) and the dandruff-associated fungi Malassezia globosa (0.39mg/mL) and Malassezia restricta (0.55mg/mL). Pronounced antioxidant activity of C. umbellata crude extract was also identified using the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay, suggesting this phytomedicine has an antioxidant function in wound healing. A chemical-genetic profile was completed with chimaphilin using approximately 4700 S. cerevisiae gene deletion mutants. Cellular roles of deleted genes in the most susceptible mutants and secondary assays indicate that the targets for chimaphilin include pathways involved in cell wall biogenesis and transcription.
    Original languageEnglish
    Pages (from-to)738-746
    JournalPhytochemistry
    Volume69
    Issue number3
    DOIs
    Publication statusPublished - 2008

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