ARA1 regulates not only l-arabinose but also d-galactose catabolism in Trichoderma reesei

Tiziano Benocci; Maria Victoria Aguilar-Pontes; Roland Sándor Kun; Bernhard Seiboth; Ronald P de Vries; Paul Daly

doi:10.1002/1873-3468.12932

ARA1 regulates not only l-arabinose but also d-galactose catabolism in Trichoderma reesei

Tiziano Benocci
, Maria Victoria Aguilar-Pontes
, Roland Sándor Kun
, Bernhard Seiboth
, Ronald P de Vries
, Paul Daly

Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
Research Area Biochemical Technology, Institute of Chemical, Environmental and Biological Engineering, TU Wien, Vienna, Austria.

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

32 Citations (Scopus)

Abstract

Trichoderma reesei is used to produce saccharifying enzyme cocktails for biofuels. There is limited understanding of the transcription factors (TFs) that regulate genes involved in release and catabolism of l-arabinose and d-galactose, as the main TF XYR1 is only partially involved. Here, the T. reesei ortholog of ARA1 from Pyricularia oryzae that regulates l-arabinose releasing and catabolic genes was deleted and characterized by growth profiling and transcriptomics along with a xyr1 mutant and xyr1/ara1 double mutant. Our results show that in addition to the l-arabinose-related role, T. reesei ARA1 is essential for expression of d-galactose releasing and catabolic genes, while XYR1 is not involved in this process.

Original language	English
Journal	FEBS Letters
DOIs	https://doi.org/10.1002/1873-3468.12932
Publication status	Published - 07 Dec 2017

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title = "ARA1 regulates not only l-arabinose but also d-galactose catabolism in Trichoderma reesei",

abstract = "Trichoderma reesei is used to produce saccharifying enzyme cocktails for biofuels. There is limited understanding of the transcription factors (TFs) that regulate genes involved in release and catabolism of l-arabinose and d-galactose, as the main TF XYR1 is only partially involved. Here, the T. reesei ortholog of ARA1 from Pyricularia oryzae that regulates l-arabinose releasing and catabolic genes was deleted and characterized by growth profiling and transcriptomics along with a xyr1 mutant and xyr1/ara1 double mutant. Our results show that in addition to the l-arabinose-related role, T. reesei ARA1 is essential for expression of d-galactose releasing and catabolic genes, while XYR1 is not involved in this process.",

keywords = "Letter",

author = "Tiziano Benocci and Aguilar-Pontes, \{Maria Victoria\} and Kun, \{Roland S{\'a}ndor\} and Bernhard Seiboth and \{de Vries\}, \{Ronald P\} and Paul Daly",

note = "{\textcopyright} 2017 Federation of European Biochemical Societies.",

year = "2017",

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day = "7",

doi = "10.1002/1873-3468.12932",

language = "English",

journal = "FEBS Letters",

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TY - JOUR

T1 - ARA1 regulates not only l-arabinose but also d-galactose catabolism in Trichoderma reesei

AU - Benocci, Tiziano

AU - Aguilar-Pontes, Maria Victoria

AU - Kun, Roland Sándor

AU - Seiboth, Bernhard

AU - de Vries, Ronald P

AU - Daly, Paul

PY - 2017/12/7

Y1 - 2017/12/7

N2 - Trichoderma reesei is used to produce saccharifying enzyme cocktails for biofuels. There is limited understanding of the transcription factors (TFs) that regulate genes involved in release and catabolism of l-arabinose and d-galactose, as the main TF XYR1 is only partially involved. Here, the T. reesei ortholog of ARA1 from Pyricularia oryzae that regulates l-arabinose releasing and catabolic genes was deleted and characterized by growth profiling and transcriptomics along with a xyr1 mutant and xyr1/ara1 double mutant. Our results show that in addition to the l-arabinose-related role, T. reesei ARA1 is essential for expression of d-galactose releasing and catabolic genes, while XYR1 is not involved in this process.

AB - Trichoderma reesei is used to produce saccharifying enzyme cocktails for biofuels. There is limited understanding of the transcription factors (TFs) that regulate genes involved in release and catabolism of l-arabinose and d-galactose, as the main TF XYR1 is only partially involved. Here, the T. reesei ortholog of ARA1 from Pyricularia oryzae that regulates l-arabinose releasing and catabolic genes was deleted and characterized by growth profiling and transcriptomics along with a xyr1 mutant and xyr1/ara1 double mutant. Our results show that in addition to the l-arabinose-related role, T. reesei ARA1 is essential for expression of d-galactose releasing and catabolic genes, while XYR1 is not involved in this process.

KW - Letter

U2 - 10.1002/1873-3468.12932

DO - 10.1002/1873-3468.12932

M3 - Article

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SN - 0014-5793

JO - FEBS Letters

JF - FEBS Letters

ER -

ARA1 regulates not only l-arabinose but also d-galactose catabolism in Trichoderma reesei

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