Aspergillus oryzae in solid-state and submerged fermentations. Progress report on a multi-disciplinary project

Rob te Biesebeke; George Ruijter; Yovita S P Rahardjo; Marisca J Hoogschagen; Margreet Heerikhuisen; Ana Levin; Kenneth G A van Driel; Maarten A I Schutyser; Jan Dijksterhuis; Yang Zhu; Frans J Weber; Willem M de Vos; Kees A M J J van den Hondel; Arjen Rinzema; Peter J Punt

Aspergillus oryzae in solid-state and submerged fermentations. Progress report on a multi-disciplinary project

Rob te Biesebeke, George Ruijter, Yovita S P Rahardjo, Marisca J Hoogschagen, Margreet Heerikhuisen, Ana Levin, Kenneth G A van Driel, Maarten A I Schutyser, Jan Dijksterhuis, Yang Zhu, Frans J Weber, Willem M de Vos, Kees A M J J van den Hondel, Arjen Rinzema, Peter J Punt

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

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Abstract

We report the progress of a multi-disciplinary research project on solid-state fermentation (SSF) of the filamentous fungus Aspergillus oryzae. The molecular and physiological aspects of the fungus in submerged fermentation (SmF) and SSF are compared and we observe a number of differences correlated with the different growth conditions. First, the aerial hyphae which occur only in SSFs are mainly responsible for oxygen uptake. Second, SSF is characterised by gradients in temperature, water activity and nutrient concentration, and inside the hyphae different polyols are accumulating. Third, pelleted growth in SmF and mycelial growth in SSF show different gene expression and protein secretion patterns. With this approach we aim to expand our knowledge of mechanisms of fungal growth on solid substrates and to exploit the biotechnological applications.

Original language	English
Pages (from-to)	245-8
Number of pages	4
Journal	FEMS Yeast Research
Volume	2
Issue number	2
Publication status	Published - May 2002

Keywords

Aspergillus oryzae
Biotechnology
Fermentation
Gene Expression Regulation, Fungal
Genes, Fungal
Hyphae
Oxygen
Oxygen Consumption

Cite this

te Biesebeke, R., Ruijter, G., Rahardjo, Y. S. P., Hoogschagen, M. J., Heerikhuisen, M., Levin, A., van Driel, K. G. A., Schutyser, M. A. I., Dijksterhuis, J., Zhu, Y., Weber, F. J., de Vos, W. M., van den Hondel, K. A. M. J. J., Rinzema, A., & Punt, P. J. (2002). Aspergillus oryzae in solid-state and submerged fermentations. Progress report on a multi-disciplinary project. FEMS Yeast Research, 2(2), 245-8.

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title = "Aspergillus oryzae in solid-state and submerged fermentations. Progress report on a multi-disciplinary project",

abstract = "We report the progress of a multi-disciplinary research project on solid-state fermentation (SSF) of the filamentous fungus Aspergillus oryzae. The molecular and physiological aspects of the fungus in submerged fermentation (SmF) and SSF are compared and we observe a number of differences correlated with the different growth conditions. First, the aerial hyphae which occur only in SSFs are mainly responsible for oxygen uptake. Second, SSF is characterised by gradients in temperature, water activity and nutrient concentration, and inside the hyphae different polyols are accumulating. Third, pelleted growth in SmF and mycelial growth in SSF show different gene expression and protein secretion patterns. With this approach we aim to expand our knowledge of mechanisms of fungal growth on solid substrates and to exploit the biotechnological applications.",

keywords = "Aspergillus oryzae, Biotechnology, Fermentation, Gene Expression Regulation, Fungal, Genes, Fungal, Hyphae, Oxygen, Oxygen Consumption",

author = "{te Biesebeke}, Rob and George Ruijter and Rahardjo, {Yovita S P} and Hoogschagen, {Marisca J} and Margreet Heerikhuisen and Ana Levin and {van Driel}, {Kenneth G A} and Schutyser, {Maarten A I} and Jan Dijksterhuis and Yang Zhu and Weber, {Frans J} and {de Vos}, {Willem M} and {van den Hondel}, {Kees A M J J} and Arjen Rinzema and Punt, {Peter J}",

year = "2002",

month = may,

language = "English",

volume = "2",

pages = "245--8",

journal = "FEMS Yeast Research",

issn = "1567-1356",

publisher = "Wiley-Blackwell",

number = "2",

}

te Biesebeke, R, Ruijter, G, Rahardjo, YSP, Hoogschagen, MJ, Heerikhuisen, M, Levin, A, van Driel, KGA, Schutyser, MAI, Dijksterhuis, J, Zhu, Y, Weber, FJ, de Vos, WM, van den Hondel, KAMJJ, Rinzema, A & Punt, PJ 2002, 'Aspergillus oryzae in solid-state and submerged fermentations. Progress report on a multi-disciplinary project', FEMS Yeast Research, vol. 2, no. 2, pp. 245-8.

TY - JOUR

T1 - Aspergillus oryzae in solid-state and submerged fermentations. Progress report on a multi-disciplinary project

AU - te Biesebeke, Rob

AU - Ruijter, George

AU - Rahardjo, Yovita S P

AU - Hoogschagen, Marisca J

AU - Heerikhuisen, Margreet

AU - Levin, Ana

AU - van Driel, Kenneth G A

AU - Schutyser, Maarten A I

AU - Dijksterhuis, Jan

AU - Zhu, Yang

AU - Weber, Frans J

AU - de Vos, Willem M

AU - van den Hondel, Kees A M J J

AU - Rinzema, Arjen

AU - Punt, Peter J

PY - 2002/5

Y1 - 2002/5

N2 - We report the progress of a multi-disciplinary research project on solid-state fermentation (SSF) of the filamentous fungus Aspergillus oryzae. The molecular and physiological aspects of the fungus in submerged fermentation (SmF) and SSF are compared and we observe a number of differences correlated with the different growth conditions. First, the aerial hyphae which occur only in SSFs are mainly responsible for oxygen uptake. Second, SSF is characterised by gradients in temperature, water activity and nutrient concentration, and inside the hyphae different polyols are accumulating. Third, pelleted growth in SmF and mycelial growth in SSF show different gene expression and protein secretion patterns. With this approach we aim to expand our knowledge of mechanisms of fungal growth on solid substrates and to exploit the biotechnological applications.

AB - We report the progress of a multi-disciplinary research project on solid-state fermentation (SSF) of the filamentous fungus Aspergillus oryzae. The molecular and physiological aspects of the fungus in submerged fermentation (SmF) and SSF are compared and we observe a number of differences correlated with the different growth conditions. First, the aerial hyphae which occur only in SSFs are mainly responsible for oxygen uptake. Second, SSF is characterised by gradients in temperature, water activity and nutrient concentration, and inside the hyphae different polyols are accumulating. Third, pelleted growth in SmF and mycelial growth in SSF show different gene expression and protein secretion patterns. With this approach we aim to expand our knowledge of mechanisms of fungal growth on solid substrates and to exploit the biotechnological applications.

KW - Aspergillus oryzae

KW - Biotechnology

KW - Fermentation

KW - Gene Expression Regulation, Fungal

KW - Genes, Fungal

KW - Hyphae

KW - Oxygen

KW - Oxygen Consumption

M3 - Article

C2 - 12702312

SN - 1567-1356

VL - 2

SP - 245

EP - 248

JO - FEMS Yeast Research

JF - FEMS Yeast Research

IS - 2

ER -

Aspergillus oryzae in solid-state and submerged fermentations. Progress report on a multi-disciplinary project

Abstract

Keywords

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