Aspergillus, Penicillium and Talaromyces isolated from house dust samples collected around the world

C M Visagie, Y Hirooka, J B Tanney, E Whitfield, K Mwange, M Meijer, A S Amend, K A Seifert, R A Samson

    Research output: Contribution to journal/periodicalArticleScientificpeer-review

    Abstract

    As part of a worldwide survey of the indoor mycobiota, dust was collected from nine countries. Analyses of dust samples included the culture-dependent dilution-to-extinction method and the culture-independent 454-pyrosequencing. Of the 7 904 isolates, 2 717 isolates were identified as belonging to Aspergillus, Penicillium and Talaromyces. The aim of this study was to identify isolates to species level and describe the new species found. Secondly, we wanted to create a reliable reference sequence database to be used for next-generation sequencing projects. Isolates represented 59 Aspergillus species, including eight undescribed species, 49 Penicillium species of which seven were undescribed and 18 Talaromyces species including three described here as new. In total, 568 ITS barcodes were generated, and 391 β-tubulin and 507 calmodulin sequences, which serve as alternative identification markers.

    Original languageEnglish
    Pages (from-to)63-139
    Number of pages77
    JournalStudies in Mycology
    Volume78
    DOIs
    Publication statusPublished - Jun 2014

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