Barcoding markers for Pneumocystis species in wildlife: Fungal Biology

P. Danesi, G. Da Rold, A. Rizzoli, H. C. Hauffe, S. Marangon, K. Samerpitak, C. Demanche, J. Guillot, G. Capelli, S. G. De Hoog

    Research output: Contribution to journal/periodicalArticleScientificpeer-review

    Abstract

    Lung specimens (n = 216) from six wildlife species were examined for occurrence of Pneumocystis species in pulmonary tissues. Among small mammals the shrew Sorex antinorii (80 %) were most frequently colonized. In contrast, foxes and badgers did not yield positive amplification. Host-specificity was noted, at least at the level of the host genus. Phylogenetic trees based on partial mtLSU and mtSSU showed high diversity of species corresponding to animal host diversity. Nuclear rDNA ITS data confirmed unambiguous separation of species. In conclusion, ITS is an excellent marker to distinguish species of the genus Pneumocystis. (C) 2015 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
    Original languageEnglish
    Pages (from-to)191-206
    Number of pages16
    JournalFungal Biology
    Volume120
    Issue number2
    DOIs
    Publication statusPublished - 2016

    Keywords

    • ITS
    • mtLSU
    • Phylogeny
    • Taxonomy

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