Biology, ecology and evolution of the family Gigasporaceae, arbuscular mycorrhizal fungi (Glomeromycota)

TY - THES

T1 - Biology, ecology and evolution of the family Gigasporaceae, arbuscular mycorrhizal fungi (Glomeromycota)

AU - De Souza, F.A.

N1 - Reporting year: 2005 Metis note: 42 NIOO Thesis; CTE; TME

PY - 2005

Y1 - 2005

N2 - Research described in this thesis focused on biological, ecological and evolutionary aspects of Arbuscular Mycorrhizal Fungi (AMF), and in particular of the family Gigasporaceae (Gigaspora and Scutellospora, genera). This study had two major objectives. The first objective was to obtain better knowledge about the life history strategies (LHS) of Gigasporaceae, and the second objective was to study the intragenomic polymorphism of the ribosomal rDNA copies in order to understand its origin and to test the use of such polymorphism to discriminate between closely related species. The LHS patterns observed for the Gigasporaceae species studied suggests that they behave like K-strategists, i.e. organisms adapted to live at stable and predictable environments, as compared to Glomeraceae species that form single spores in the soil. Based on LHS patterns of Gigasporaceae an hypotheses that those species will be negatively selected in disturbed ecosystems have been formulated. Although intraspecific rDNA polymorphism in Gigasporaceae has been previously reported, this study, for the first time, systematically describes this heterogeneity and analyses this phenomenon with respect to Gigasporaceae phylogeny and evolution. It has been demonstrated that the intragenomic polymorphism of the rDNA copies can be used to differentiate species and in the case of Gigaspora even isolates. The phylogenetic analysis of inter and intragenomic rDNA polymorphism of closely related Gigaspora species strongly suggested that reticulated evolution, characterized by occasional hybridization or horizontal gene transfer between two species, played a significant role in evolution of this asexual group of fungi, via parasexual recombination. In addition, a molecular method was developed to assess Gigasporaceae diversity in environmental samples

AB - Research described in this thesis focused on biological, ecological and evolutionary aspects of Arbuscular Mycorrhizal Fungi (AMF), and in particular of the family Gigasporaceae (Gigaspora and Scutellospora, genera). This study had two major objectives. The first objective was to obtain better knowledge about the life history strategies (LHS) of Gigasporaceae, and the second objective was to study the intragenomic polymorphism of the ribosomal rDNA copies in order to understand its origin and to test the use of such polymorphism to discriminate between closely related species. The LHS patterns observed for the Gigasporaceae species studied suggests that they behave like K-strategists, i.e. organisms adapted to live at stable and predictable environments, as compared to Glomeraceae species that form single spores in the soil. Based on LHS patterns of Gigasporaceae an hypotheses that those species will be negatively selected in disturbed ecosystems have been formulated. Although intraspecific rDNA polymorphism in Gigasporaceae has been previously reported, this study, for the first time, systematically describes this heterogeneity and analyses this phenomenon with respect to Gigasporaceae phylogeny and evolution. It has been demonstrated that the intragenomic polymorphism of the rDNA copies can be used to differentiate species and in the case of Gigaspora even isolates. The phylogenetic analysis of inter and intragenomic rDNA polymorphism of closely related Gigaspora species strongly suggested that reticulated evolution, characterized by occasional hybridization or horizontal gene transfer between two species, played a significant role in evolution of this asexual group of fungi, via parasexual recombination. In addition, a molecular method was developed to assess Gigasporaceae diversity in environmental samples

M3 - PhD thesis (Proefschrift)

PB - Rijksuniversiteit Leiden

CY - Leiden

ER -