Black yeasts-like fungi isolated from dialysis water in hemodialysis units

Izabel Cristina Figel; Paulo Roberto Dantas Marangoni; Sabina Moser Tralamazza; Vânia Aparecida Vicente; Patrícia do Rocio Dalzoto; Mariana Machado Fidelis do Nascimento; G Sybren de Hoog; Ida Chapaval Pimentel

doi:10.1007/s11046-013-9633-4

Black yeasts-like fungi isolated from dialysis water in hemodialysis units

Izabel Cristina Figel, Paulo Roberto Dantas Marangoni, Sabina Moser Tralamazza, Vânia Aparecida Vicente, Patrícia do Rocio Dalzoto, Mariana Machado Fidelis do Nascimento, G Sybren de Hoog, Ida Chapaval Pimentel

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

24 Citations (Scopus)

Abstract

Hemodialysis in patients with chronic renal failure promotes the removal of toxic substances, water, and minerals from the body and often takes place in specialized clinics. Microbial contamination of dialysis fluid is a serious problem in therapy. One of the sources of contamination is the water used to prepare the dialysate. In Brazil, legislation regulating the microbiological quality of water for dialysis does not cover waterborne microbes such as Pseudomonas, mycobacteria, and fungi. The aim of the present study was to quantify, isolate, and identify fungi present in water systems in six hemodialysis units in Curitiba, Parana state, Brazil. Fungi were analyzed by surface plating and membrane filtration. Isolates were identified by morphology, while the dematiaceous fungi were identified by sequencing the rDNA ITS region. It was found that 66 % of the samples presented fungi, while black fungi were present in 46 % of all samples. Twenty-eight isolates from treated water for dialysis and dialysate were identified by sequencing and were found to be Exophiala pisciphila, E. cancerae, E. equina, and Rhinocladiella similis. The presence of dematiaceous fungi may pose a risk for debilitated hospitalized patients.

Original language	English
Pages (from-to)	413-420
Number of pages	8
Journal	Mycopathologia
Volume	175
Issue number	5-6
DOIs	https://doi.org/10.1007/s11046-013-9633-4
Publication status	Published - Jun 2013

Keywords

Biodiversity
Brazil
DNA, Fungal
DNA, Ribosomal Spacer
Fungi
Hemodialysis Units, Hospital
Humans
Molecular Sequence Data
Sequence Analysis, DNA
Water Microbiology

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title = "Black yeasts-like fungi isolated from dialysis water in hemodialysis units",

abstract = "Hemodialysis in patients with chronic renal failure promotes the removal of toxic substances, water, and minerals from the body and often takes place in specialized clinics. Microbial contamination of dialysis fluid is a serious problem in therapy. One of the sources of contamination is the water used to prepare the dialysate. In Brazil, legislation regulating the microbiological quality of water for dialysis does not cover waterborne microbes such as Pseudomonas, mycobacteria, and fungi. The aim of the present study was to quantify, isolate, and identify fungi present in water systems in six hemodialysis units in Curitiba, Parana state, Brazil. Fungi were analyzed by surface plating and membrane filtration. Isolates were identified by morphology, while the dematiaceous fungi were identified by sequencing the rDNA ITS region. It was found that 66 % of the samples presented fungi, while black fungi were present in 46 % of all samples. Twenty-eight isolates from treated water for dialysis and dialysate were identified by sequencing and were found to be Exophiala pisciphila, E. cancerae, E. equina, and Rhinocladiella similis. The presence of dematiaceous fungi may pose a risk for debilitated hospitalized patients.",

keywords = "Biodiversity, Brazil, DNA, Fungal, DNA, Ribosomal Spacer, Fungi, Hemodialysis Units, Hospital, Humans, Molecular Sequence Data, Sequence Analysis, DNA, Water Microbiology",

author = "Figel, {Izabel Cristina} and Marangoni, {Paulo Roberto Dantas} and Tralamazza, {Sabina Moser} and Vicente, {V{\^a}nia Aparecida} and Dalzoto, {Patr{\'i}cia do Rocio} and {do Nascimento}, {Mariana Machado Fidelis} and {de Hoog}, {G Sybren} and Pimentel, {Ida Chapaval}",

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journal = "Mycopathologia",

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AU - Figel, Izabel Cristina

AU - Marangoni, Paulo Roberto Dantas

AU - Tralamazza, Sabina Moser

AU - Vicente, Vânia Aparecida

AU - Dalzoto, Patrícia do Rocio

AU - do Nascimento, Mariana Machado Fidelis

AU - de Hoog, G Sybren

AU - Pimentel, Ida Chapaval

N1 - Reporting year: 2013

PY - 2013/6

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N2 - Hemodialysis in patients with chronic renal failure promotes the removal of toxic substances, water, and minerals from the body and often takes place in specialized clinics. Microbial contamination of dialysis fluid is a serious problem in therapy. One of the sources of contamination is the water used to prepare the dialysate. In Brazil, legislation regulating the microbiological quality of water for dialysis does not cover waterborne microbes such as Pseudomonas, mycobacteria, and fungi. The aim of the present study was to quantify, isolate, and identify fungi present in water systems in six hemodialysis units in Curitiba, Parana state, Brazil. Fungi were analyzed by surface plating and membrane filtration. Isolates were identified by morphology, while the dematiaceous fungi were identified by sequencing the rDNA ITS region. It was found that 66 % of the samples presented fungi, while black fungi were present in 46 % of all samples. Twenty-eight isolates from treated water for dialysis and dialysate were identified by sequencing and were found to be Exophiala pisciphila, E. cancerae, E. equina, and Rhinocladiella similis. The presence of dematiaceous fungi may pose a risk for debilitated hospitalized patients.

AB - Hemodialysis in patients with chronic renal failure promotes the removal of toxic substances, water, and minerals from the body and often takes place in specialized clinics. Microbial contamination of dialysis fluid is a serious problem in therapy. One of the sources of contamination is the water used to prepare the dialysate. In Brazil, legislation regulating the microbiological quality of water for dialysis does not cover waterborne microbes such as Pseudomonas, mycobacteria, and fungi. The aim of the present study was to quantify, isolate, and identify fungi present in water systems in six hemodialysis units in Curitiba, Parana state, Brazil. Fungi were analyzed by surface plating and membrane filtration. Isolates were identified by morphology, while the dematiaceous fungi were identified by sequencing the rDNA ITS region. It was found that 66 % of the samples presented fungi, while black fungi were present in 46 % of all samples. Twenty-eight isolates from treated water for dialysis and dialysate were identified by sequencing and were found to be Exophiala pisciphila, E. cancerae, E. equina, and Rhinocladiella similis. The presence of dematiaceous fungi may pose a risk for debilitated hospitalized patients.

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KW - Brazil

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KW - DNA, Ribosomal Spacer

KW - Fungi

KW - Hemodialysis Units, Hospital

KW - Humans

KW - Molecular Sequence Data

KW - Sequence Analysis, DNA

KW - Water Microbiology

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JO - Mycopathologia

JF - Mycopathologia

IS - 5-6

ER -

Black yeasts-like fungi isolated from dialysis water in hemodialysis units

Abstract

Keywords

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