Black yeasts-like fungi isolated from dialysis water in hemodialysis units

Izabel Cristina Figel, Paulo Roberto Dantas Marangoni, Sabina Moser Tralamazza, Vânia Aparecida Vicente, Patrícia do Rocio Dalzoto, Mariana Machado Fidelis do Nascimento, G Sybren de Hoog, Ida Chapaval Pimentel

    Research output: Contribution to journal/periodicalArticleScientificpeer-review

    Abstract

    Hemodialysis in patients with chronic renal failure promotes the removal of toxic substances, water, and minerals from the body and often takes place in specialized clinics. Microbial contamination of dialysis fluid is a serious problem in therapy. One of the sources of contamination is the water used to prepare the dialysate. In Brazil, legislation regulating the microbiological quality of water for dialysis does not cover waterborne microbes such as Pseudomonas, mycobacteria, and fungi. The aim of the present study was to quantify, isolate, and identify fungi present in water systems in six hemodialysis units in Curitiba, Parana state, Brazil. Fungi were analyzed by surface plating and membrane filtration. Isolates were identified by morphology, while the dematiaceous fungi were identified by sequencing the rDNA ITS region. It was found that 66 % of the samples presented fungi, while black fungi were present in 46 % of all samples. Twenty-eight isolates from treated water for dialysis and dialysate were identified by sequencing and were found to be Exophiala pisciphila, E. cancerae, E. equina, and Rhinocladiella similis. The presence of dematiaceous fungi may pose a risk for debilitated hospitalized patients.
    Original languageEnglish
    Pages (from-to)413-420
    Number of pages8
    JournalMycopathologia
    Volume175
    Issue number5-6
    DOIs
    Publication statusPublished - Jun 2013

    Keywords

    • Biodiversity
    • Brazil
    • DNA, Fungal
    • DNA, Ribosomal Spacer
    • Fungi
    • Hemodialysis Units, Hospital
    • Humans
    • Molecular Sequence Data
    • Sequence Analysis, DNA
    • Water Microbiology

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