Bradymyces gen. nov (Chaetothyriales, Trichomeriaceae): a new ascomycete genus accommodating poorly differentiated melanized fungi

Vit Hubka, Martina Reblova, Jiri Rehulka, Laura Selbmann, Daniela Isola, Sybren G. de Hoog, Miroslav Kolarik

    Research output: Contribution to journal/periodicalArticleScientificpeer-review

    Abstract

    Three slow growing, melanized and morphologically poorly differentiated fungal strains were isolated from a hyperaemic focus near the enlarged spleen of a farmed rainbow trout (Oncorhynchus mykiss) and from a rock collected at 3,200 m a. s. l. (Alps, Italy). Two phylogenetic analyses of the combined nuc18S and nuc28S rDNA and ITS rDNA and β-tubulin sequences showed that these isolates belong to the Trichomeriaceae, a family of the ascomycete order Chaetothyriales containing black yeasts that cause infections in humans and animals. The strains form a well-supported monophyletic clade. The new genus Bradymyces, with two new species, Bradymyces oncorhynchi and Bradymyces alpinus, is proposed based on phylogenetic, ecophysiological and morphological data. It is characterized by the presence of moniliform hyphae, blastic proliferation, endoconidia, multicellular and muriform bodies, and bodies with dark fragmented incrustations on the surface. Bradymyces most closely resembles members of Knufia. The ex-type isolate of B. oncorhynchi CCF 4369T ( = CBS 133066T = CCFEE 6134T) represents the first case of a Trichomeriaceae member isolated from cold-blooded water vertebrates. B. alpinus [ex-type strain CCFEE 5493T ( = CBS 138368T = CCF 4803T)] is represented by two isolates from a single locality in the Alps and in contrast to B. oncorhynchi shows overall slower growth parameters and does not grow at 25 °C.
    Original languageEnglish
    Pages (from-to)979-992
    JournalAntonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
    Volume106
    Issue number5
    DOIs
    Publication statusPublished - Nov 2014

    Keywords

    • Black yeast
    • Extremophilic fungi
    • Farmed rainbow trout
    • Oncorhynchus mykiss
    • Multi-locus phylogeny
    • Rock-inhabiting fungi

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