Casing soil microbiome mediates suppression of bacterial blotch of mushrooms during consecutive cultivation cycles

Tanvi Taparia; Ed Hendrix; Marc Hendriks; Els Nijhuis; Wietse de Boer; Jan van der Wolf

doi:10.1016/j.soilbio.2021.108161

Casing soil microbiome mediates suppression of bacterial blotch of mushrooms during consecutive cultivation cycles

Tanvi Taparia^* (Corresponding author), Ed Hendrix, Marc Hendriks, Els Nijhuis, Wietse de Boer, Jan van der Wolf (Corresponding author)

^*Corresponding author for this work

NIOO - Microbial Ecology (ME)

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

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Abstract

Shifts in the soil microbiome during continuous monoculture cropping coincide with increased suppressiveness against soil-borne diseases, as in the take-all decline of wheat. Here we report a similar phenomenon for bacterial blotch of mushrooms, caused by Pseudomonas ‘gingeri’, where ginger blotch incidence decreases during consecutive cycles of mushroom cultivation. We explored the infection dynamics of blotch during consecutive cultivation cycles for different casing soil mixtures. We also observed the population dynamics of the pathogen in these casing soils. In addition, the composition of the casing soil microbiome was compared between blotch suppressive and conducive soils. Finally, we studied the transferability of blotch suppressiveness. A consistent decline of bacterial blotch was observed for two consecutive cultivation cycles of mushroom cropping, across ten casing soil mixtures composed of different peat sources and supplements. Blotch suppression occurred without reduction of pathogen populations in the casing soils. Aqueous extracts made from suppressive soils were able to reduce blotch incidence in conducive casing soils, indicating that blotch suppression is transferrable and microbially mediated. Changes in the microbial community composition of the casing soils reflected pathogen invasion, pathogen establishment and disease suppression, in addition to the expected temporal changes across the cultivation cycles. Specific bacterial genera were associated with soil suppressiveness to bacterial blotch, such as, Pseudomonas sp., Dyadobacter sp., Pedobacter sp., and Flavobacterium sp. We suggest that the suppression of bacterial blotch is induced due to high pathogen populations in the first cultivation cycle, and mediated by inhibition of virulence factors such as those controlled by quorum sensing in the later cultivation cycles.

Original language	English
Article number	108161
Journal	Soil Biology and Biochemistry
Volume	155
DOIs	https://doi.org/10.1016/j.soilbio.2021.108161
Publication status	Published - Apr 2021

Keywords

Agaricus bisporus
Microbial ecology
Pathogen populations
Pseudomonas ‘gingeri’
Soil-borne pathogens
TaqmanTM-qPCR
national
Plan_S-Compliant_OA

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title = "Casing soil microbiome mediates suppression of bacterial blotch of mushrooms during consecutive cultivation cycles",

abstract = "Shifts in the soil microbiome during continuous monoculture cropping coincide with increased suppressiveness against soil-borne diseases, as in the take-all decline of wheat. Here we report a similar phenomenon for bacterial blotch of mushrooms, caused by Pseudomonas {\textquoteleft}gingeri{\textquoteright}, where ginger blotch incidence decreases during consecutive cycles of mushroom cultivation. We explored the infection dynamics of blotch during consecutive cultivation cycles for different casing soil mixtures. We also observed the population dynamics of the pathogen in these casing soils. In addition, the composition of the casing soil microbiome was compared between blotch suppressive and conducive soils. Finally, we studied the transferability of blotch suppressiveness. A consistent decline of bacterial blotch was observed for two consecutive cultivation cycles of mushroom cropping, across ten casing soil mixtures composed of different peat sources and supplements. Blotch suppression occurred without reduction of pathogen populations in the casing soils. Aqueous extracts made from suppressive soils were able to reduce blotch incidence in conducive casing soils, indicating that blotch suppression is transferrable and microbially mediated. Changes in the microbial community composition of the casing soils reflected pathogen invasion, pathogen establishment and disease suppression, in addition to the expected temporal changes across the cultivation cycles. Specific bacterial genera were associated with soil suppressiveness to bacterial blotch, such as, Pseudomonas sp., Dyadobacter sp., Pedobacter sp., and Flavobacterium sp. We suggest that the suppression of bacterial blotch is induced due to high pathogen populations in the first cultivation cycle, and mediated by inhibition of virulence factors such as those controlled by quorum sensing in the later cultivation cycles.",

keywords = "Agaricus bisporus, Microbial ecology, Pathogen populations, Pseudomonas {\textquoteleft}gingeri{\textquoteright}, Soil-borne pathogens, TaqmanTM-qPCR, national, Plan_S-Compliant_OA",

author = "Tanvi Taparia and Ed Hendrix and Marc Hendriks and Els Nijhuis and {de Boer}, Wietse and {van der Wolf}, Jan",

note = "7161, ME; Data archiving: property of WUR",

year = "2021",

month = apr,

doi = "10.1016/j.soilbio.2021.108161",

language = "English",

volume = "155",

journal = "Soil Biology and Biochemistry",

issn = "0038-0717",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Casing soil microbiome mediates suppression of bacterial blotch of mushrooms during consecutive cultivation cycles

AU - Taparia, Tanvi

AU - Hendrix, Ed

AU - Hendriks, Marc

AU - Nijhuis, Els

AU - de Boer, Wietse

AU - van der Wolf, Jan

N1 - 7161, ME; Data archiving: property of WUR

PY - 2021/4

Y1 - 2021/4

N2 - Shifts in the soil microbiome during continuous monoculture cropping coincide with increased suppressiveness against soil-borne diseases, as in the take-all decline of wheat. Here we report a similar phenomenon for bacterial blotch of mushrooms, caused by Pseudomonas ‘gingeri’, where ginger blotch incidence decreases during consecutive cycles of mushroom cultivation. We explored the infection dynamics of blotch during consecutive cultivation cycles for different casing soil mixtures. We also observed the population dynamics of the pathogen in these casing soils. In addition, the composition of the casing soil microbiome was compared between blotch suppressive and conducive soils. Finally, we studied the transferability of blotch suppressiveness. A consistent decline of bacterial blotch was observed for two consecutive cultivation cycles of mushroom cropping, across ten casing soil mixtures composed of different peat sources and supplements. Blotch suppression occurred without reduction of pathogen populations in the casing soils. Aqueous extracts made from suppressive soils were able to reduce blotch incidence in conducive casing soils, indicating that blotch suppression is transferrable and microbially mediated. Changes in the microbial community composition of the casing soils reflected pathogen invasion, pathogen establishment and disease suppression, in addition to the expected temporal changes across the cultivation cycles. Specific bacterial genera were associated with soil suppressiveness to bacterial blotch, such as, Pseudomonas sp., Dyadobacter sp., Pedobacter sp., and Flavobacterium sp. We suggest that the suppression of bacterial blotch is induced due to high pathogen populations in the first cultivation cycle, and mediated by inhibition of virulence factors such as those controlled by quorum sensing in the later cultivation cycles.

AB - Shifts in the soil microbiome during continuous monoculture cropping coincide with increased suppressiveness against soil-borne diseases, as in the take-all decline of wheat. Here we report a similar phenomenon for bacterial blotch of mushrooms, caused by Pseudomonas ‘gingeri’, where ginger blotch incidence decreases during consecutive cycles of mushroom cultivation. We explored the infection dynamics of blotch during consecutive cultivation cycles for different casing soil mixtures. We also observed the population dynamics of the pathogen in these casing soils. In addition, the composition of the casing soil microbiome was compared between blotch suppressive and conducive soils. Finally, we studied the transferability of blotch suppressiveness. A consistent decline of bacterial blotch was observed for two consecutive cultivation cycles of mushroom cropping, across ten casing soil mixtures composed of different peat sources and supplements. Blotch suppression occurred without reduction of pathogen populations in the casing soils. Aqueous extracts made from suppressive soils were able to reduce blotch incidence in conducive casing soils, indicating that blotch suppression is transferrable and microbially mediated. Changes in the microbial community composition of the casing soils reflected pathogen invasion, pathogen establishment and disease suppression, in addition to the expected temporal changes across the cultivation cycles. Specific bacterial genera were associated with soil suppressiveness to bacterial blotch, such as, Pseudomonas sp., Dyadobacter sp., Pedobacter sp., and Flavobacterium sp. We suggest that the suppression of bacterial blotch is induced due to high pathogen populations in the first cultivation cycle, and mediated by inhibition of virulence factors such as those controlled by quorum sensing in the later cultivation cycles.

KW - Agaricus bisporus

KW - Microbial ecology

KW - Pathogen populations

KW - Pseudomonas ‘gingeri’

KW - Soil-borne pathogens

KW - TaqmanTM-qPCR

KW - national

KW - Plan_S-Compliant_OA

U2 - 10.1016/j.soilbio.2021.108161

DO - 10.1016/j.soilbio.2021.108161

M3 - Article

AN - SCOPUS:85101004741

SN - 0038-0717

VL - 155

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

M1 - 108161

ER -

Casing soil microbiome mediates suppression of bacterial blotch of mushrooms during consecutive cultivation cycles

Abstract

Keywords

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