Cell entry of a host-targeting protein of oomycetes requires gp96

TY - JOUR

T1 - Cell entry of a host-targeting protein of oomycetes requires gp96

AU - Trusch, F.

AU - Loebach, L.

AU - Wawra, S.

AU - Durward, E.

AU - Wuensch, A.

AU - Iberahim, Nurul Aqilah

AU - De Bruijn, I.

AU - MacKenzie, K.

AU - Willems, A.

AU - Toloczkoa, A.

AU - Diéguez-Uribeondo, J.

AU - Rasmussen, T.

AU - Schraderf, T.

AU - Bayer, P.

AU - Secombes, C.J.

AU - van West, P.

N1 - 6550, ME; Data archiving: data archived at University of Aberdeen

PY - 2018

Y1 - 2018

N2 - The animal-pathogenic oomycete Saprolegnia parasitica causes serious losses in aquaculture by infecting and killing freshwater fish. Like plant-pathogenic oomycetes, S. parasitica employs similar infection structures and secretes effector proteins that translocate into host cells to manipulate the host. Here, we show that the host-targeting protein SpHtp3 enters fish cells in a pathogen-independent manner. This uptake process is guided by a gp96-like receptor and can be inhibited by supramolecular tweezers. The C-terminus of SpHtp3 (containing the amino acid sequence YKARK), and not the N-terminal RxLR motif, is responsible for the uptake into host cells. Following translocation, SpHtp3 is released from vesicles into the cytoplasm by another host-targeting protein where it degrades nucleic acids. The effector translocation mechanism described here, is potentially also relevant for other pathogen–host interactions as gp96 is found in both animals and plants.

AB - The animal-pathogenic oomycete Saprolegnia parasitica causes serious losses in aquaculture by infecting and killing freshwater fish. Like plant-pathogenic oomycetes, S. parasitica employs similar infection structures and secretes effector proteins that translocate into host cells to manipulate the host. Here, we show that the host-targeting protein SpHtp3 enters fish cells in a pathogen-independent manner. This uptake process is guided by a gp96-like receptor and can be inhibited by supramolecular tweezers. The C-terminus of SpHtp3 (containing the amino acid sequence YKARK), and not the N-terminal RxLR motif, is responsible for the uptake into host cells. Following translocation, SpHtp3 is released from vesicles into the cytoplasm by another host-targeting protein where it degrades nucleic acids. The effector translocation mechanism described here, is potentially also relevant for other pathogen–host interactions as gp96 is found in both animals and plants.

KW - international

U2 - 10.1038/s41467-018-04796-3

DO - 10.1038/s41467-018-04796-3

M3 - Article

SN - 2041-1723

VL - 9

JO - Nature Communications

JF - Nature Communications

M1 - 2347

ER -