Characterization and biotechnological application of recombinant xylanases from Aspergillus nidulans

Gabriela P Maitan-Alfenas, Mariana B Oliveira, Ronaldo A P Nagem, Ronald P de Vries, Valéria M Guimarães

    Research output: Contribution to journal/periodicalArticleScientificpeer-review

    Abstract

    Two xylanases from Aspergillus nidulans, XlnB and XlnC, were expressed in Pichia pastoris, purified and characterized. XlnB and XlnC achieved maximal activities at 60°C and pH 7.5 and at 50°C and pH 6.0, respectively. XlnB showed to be very thermostable by maintaining 50% of its original activity after 49h incubated at 50°C. XlnB had its highest activity against wheat arabinoxylan while XlnC had the best activity against beechwood xylan. Both enzymes were completely inhibited by SDS and HgCl2. Xylotriose at 1mg/ml also totally inibited XlnB activity. TLC analysis showed that the main product of beechwood xylan hydrolysis by XlnB and XlnC was xylotetraose. An additive effect was shown between XlnB and XlnC and the xylanases of two tested commercial cocktails. Sugarcane bagasse saccharification results showed that these two commercial enzymatic cocktails were able to release more glucose and xylose after supplementation with XlnB and XlnC.

    Original languageEnglish
    Pages (from-to)60-67
    Number of pages8
    JournalInternational Journal of Biological Macromolecules
    Volume91
    DOIs
    Publication statusPublished - 25 May 2016

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