Characterization of d-xylose reductase, XyrB, from Aspergillus niger

Agata Terebieniec, Tania Chroumpi, Adiphol Dilokpimol, Maria Victoria Aguilar-Pontes, Miia R Mäkelä, Ronald P de Vries

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

d-xylose reductase is a member of the aldo-keto reductase family, and is involved in d-xylose and l-arabinose conversion through the Pentose Catabolic Pathway (PCP) in fungi. In this study, we biochemically characterized a newly identified second d-xylose reductase (XyrB) from Aspergillus niger. This NADPH-dependent reductase is able to efficiently convert d-xylose and l-arabinose, and it has the highest affinity for these sugars of all currently known fungal pentose reductases. A combination of biochemical data, transcriptomics and phylogenetic analysis further illustrated the role of XyrB in the PCP. Enzymes: D-xylose reductase (EC 1.1.1.307), L-arabinose reductase (EC 1.1.1.21).

Original languageEnglish
Pages (from-to)e00610
JournalBiotechnology reports (Amsterdam, Netherlands)
Volume30
DOIs
Publication statusPublished - Jun 2021

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