Characterization of d-xylose reductase, XyrB, from Aspergillus niger

Agata Terebieniec; Tania Chroumpi; Adiphol Dilokpimol; Maria Victoria Aguilar-Pontes; Miia R Mäkelä; Ronald P de Vries

doi:10.1016/j.btre.2021.e00610

Characterization of d-xylose reductase, XyrB, from Aspergillus niger

Agata Terebieniec, Tania Chroumpi, Adiphol Dilokpimol, Maria Victoria Aguilar-Pontes, Miia R Mäkelä, Ronald P de Vries

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

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Abstract

d-xylose reductase is a member of the aldo-keto reductase family, and is involved in d-xylose and l-arabinose conversion through the Pentose Catabolic Pathway (PCP) in fungi. In this study, we biochemically characterized a newly identified second d-xylose reductase (XyrB) from Aspergillus niger. This NADPH-dependent reductase is able to efficiently convert d-xylose and l-arabinose, and it has the highest affinity for these sugars of all currently known fungal pentose reductases. A combination of biochemical data, transcriptomics and phylogenetic analysis further illustrated the role of XyrB in the PCP. Enzymes: D-xylose reductase (EC 1.1.1.307), L-arabinose reductase (EC 1.1.1.21).

Original language	English
Pages (from-to)	e00610
Journal	Biotechnology reports (Amsterdam, Netherlands)
Volume	30
DOIs	https://doi.org/10.1016/j.btre.2021.e00610
Publication status	Published - Jun 2021

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title = "Characterization of d-xylose reductase, XyrB, from Aspergillus niger",

abstract = "d-xylose reductase is a member of the aldo-keto reductase family, and is involved in d-xylose and l-arabinose conversion through the Pentose Catabolic Pathway (PCP) in fungi. In this study, we biochemically characterized a newly identified second d-xylose reductase (XyrB) from Aspergillus niger. This NADPH-dependent reductase is able to efficiently convert d-xylose and l-arabinose, and it has the highest affinity for these sugars of all currently known fungal pentose reductases. A combination of biochemical data, transcriptomics and phylogenetic analysis further illustrated the role of XyrB in the PCP. Enzymes: D-xylose reductase (EC 1.1.1.307), L-arabinose reductase (EC 1.1.1.21).",

author = "Agata Terebieniec and Tania Chroumpi and Adiphol Dilokpimol and Aguilar-Pontes, {Maria Victoria} and M{\"a}kel{\"a}, {Miia R} and {de Vries}, {Ronald P}",

note = "{\textcopyright} 2021 The Authors.",

year = "2021",

month = jun,

doi = "10.1016/j.btre.2021.e00610",

language = "English",

volume = "30",

pages = "e00610",

journal = "Biotechnology reports (Amsterdam, Netherlands)",

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TY - JOUR

T1 - Characterization of d-xylose reductase, XyrB, from Aspergillus niger

AU - Terebieniec, Agata

AU - Chroumpi, Tania

AU - Dilokpimol, Adiphol

AU - Aguilar-Pontes, Maria Victoria

AU - Mäkelä, Miia R

AU - de Vries, Ronald P

PY - 2021/6

Y1 - 2021/6

N2 - d-xylose reductase is a member of the aldo-keto reductase family, and is involved in d-xylose and l-arabinose conversion through the Pentose Catabolic Pathway (PCP) in fungi. In this study, we biochemically characterized a newly identified second d-xylose reductase (XyrB) from Aspergillus niger. This NADPH-dependent reductase is able to efficiently convert d-xylose and l-arabinose, and it has the highest affinity for these sugars of all currently known fungal pentose reductases. A combination of biochemical data, transcriptomics and phylogenetic analysis further illustrated the role of XyrB in the PCP. Enzymes: D-xylose reductase (EC 1.1.1.307), L-arabinose reductase (EC 1.1.1.21).

AB - d-xylose reductase is a member of the aldo-keto reductase family, and is involved in d-xylose and l-arabinose conversion through the Pentose Catabolic Pathway (PCP) in fungi. In this study, we biochemically characterized a newly identified second d-xylose reductase (XyrB) from Aspergillus niger. This NADPH-dependent reductase is able to efficiently convert d-xylose and l-arabinose, and it has the highest affinity for these sugars of all currently known fungal pentose reductases. A combination of biochemical data, transcriptomics and phylogenetic analysis further illustrated the role of XyrB in the PCP. Enzymes: D-xylose reductase (EC 1.1.1.307), L-arabinose reductase (EC 1.1.1.21).

U2 - 10.1016/j.btre.2021.e00610

DO - 10.1016/j.btre.2021.e00610

M3 - Article

C2 - 33842213

SN - 2215-017X

VL - 30

SP - e00610

JO - Biotechnology reports (Amsterdam, Netherlands)

JF - Biotechnology reports (Amsterdam, Netherlands)

ER -

Characterization of d-xylose reductase, XyrB, from Aspergillus niger

Abstract

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