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Chitosan as coagulant on cyanobacteria in lake restoration management may cause rapid cell lysis. / Mucci, Maira (Corresponding author); Noyma, Natalia Pessoa; de Magalhaes, Leonardo; Miranda, Marcela; van Oosterhout, Frank; Guedes, Iame Alves; Huszar, Vera L. M.; Marinho, Marcelo Manzi; Lürling, Miquel.

In: Water Research, Vol. 118, 01.07.2017, p. 121-130.

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Harvard

Mucci, M, Noyma, NP, de Magalhaes, L, Miranda, M, van Oosterhout, F, Guedes, IA, Huszar, VLM, Marinho, MM & Lürling, M 2017, 'Chitosan as coagulant on cyanobacteria in lake restoration management may cause rapid cell lysis' Water Research, vol. 118, pp. 121-130. DOI: 10.1016/j.watres.2017.04.020

APA

Mucci, M., Noyma, N. P., de Magalhaes, L., Miranda, M., van Oosterhout, F., Guedes, I. A., ... Lürling, M. (2017). Chitosan as coagulant on cyanobacteria in lake restoration management may cause rapid cell lysis. DOI: 10.1016/j.watres.2017.04.020

Vancouver

Mucci M, Noyma NP, de Magalhaes L, Miranda M, van Oosterhout F, Guedes IA et al. Chitosan as coagulant on cyanobacteria in lake restoration management may cause rapid cell lysis. Water Research. 2017 Jul 1;118:121-130. Available from, DOI: 10.1016/j.watres.2017.04.020

Author

Mucci, Maira ; Noyma, Natalia Pessoa ; de Magalhaes, Leonardo ; Miranda, Marcela ; van Oosterhout, Frank ; Guedes, Iame Alves ; Huszar, Vera L. M. ; Marinho, Marcelo Manzi ; Lürling, Miquel. / Chitosan as coagulant on cyanobacteria in lake restoration management may cause rapid cell lysis. In: Water Research. 2017 ; Vol. 118. pp. 121-130

BibTeX

@article{0c41218890274e7b819a503244129e5a,
title = "Chitosan as coagulant on cyanobacteria in lake restoration management may cause rapid cell lysis",
abstract = "Combining coagulant and ballast to remove cyanobacteria from the water column is a promising restoration technique to mitigate cyanobacterial nuisance in surface waters. The organic, biodegradable polymer chitosan has been promoted as a coagulant and is viewed as non-toxic. In this study, we show that chitosan may rapidly compromise membrane integrity and kill certain cyanobacteria leading to release of cell contents in the water. A strain of Cylindrospermopsis raciborskii and one strain of Planktothrix agardhii were most sensitive. A 1.3 h exposure to a low dose of 0.5 mg l−1 chitosan already almost completely killed these cultures resulting in release of cell contents. After 24 h, reductions in PSII efficiencies of all cyanobacteria tested were observed. EC50 values varied from around 0.5 mg l−1 chitosan for the two sensitive strains, via about 5 mg l−1 chitosan for an Aphanizomenon flos-aquae strain, a toxic P. agardhii strain and two Anabaena cylindrica cultures, to more than 8 mg l−1 chitosan for a Microcystis aeruginosa strain and another A. flos-aquae strain. Differences in sensitivity to chitosan might be related to polymeric substances that surround cyanobacteria. Rapid lysis of toxic strains is likely and when chitosan flocking and sinking of cyanobacteria is considered in lake restoration, flocculation efficacy studies should be complemented with investigation on the effects of chitosan on the cyanobacteria assemblage being targeted.",
keywords = "Cell lysis, Cell viability, Cyanobacterial blooms, Eutrophication, Lake restoration, Photosystem II efficiency, international",
author = "Maira Mucci and Noyma, {Natalia Pessoa} and {de Magalhaes}, Leonardo and Marcela Miranda and {van Oosterhout}, Frank and Guedes, {Iame Alves} and Huszar, {Vera L. M.} and Marinho, {Marcelo Manzi} and Miquel L{\"u}rling",
note = "6317, AqE; Data archiving: data property of and archived at WUR",
year = "2017",
month = "7",
day = "1",
doi = "10.1016/j.watres.2017.04.020",
language = "English",
volume = "118",
pages = "121--130",
journal = "Water Research",
issn = "0043-1354",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Chitosan as coagulant on cyanobacteria in lake restoration management may cause rapid cell lysis

AU - Mucci,Maira

AU - Noyma,Natalia Pessoa

AU - de Magalhaes,Leonardo

AU - Miranda,Marcela

AU - van Oosterhout,Frank

AU - Guedes,Iame Alves

AU - Huszar,Vera L. M.

AU - Marinho,Marcelo Manzi

AU - Lürling,Miquel

N1 - 6317, AqE; Data archiving: data property of and archived at WUR

PY - 2017/7/1

Y1 - 2017/7/1

N2 - Combining coagulant and ballast to remove cyanobacteria from the water column is a promising restoration technique to mitigate cyanobacterial nuisance in surface waters. The organic, biodegradable polymer chitosan has been promoted as a coagulant and is viewed as non-toxic. In this study, we show that chitosan may rapidly compromise membrane integrity and kill certain cyanobacteria leading to release of cell contents in the water. A strain of Cylindrospermopsis raciborskii and one strain of Planktothrix agardhii were most sensitive. A 1.3 h exposure to a low dose of 0.5 mg l−1 chitosan already almost completely killed these cultures resulting in release of cell contents. After 24 h, reductions in PSII efficiencies of all cyanobacteria tested were observed. EC50 values varied from around 0.5 mg l−1 chitosan for the two sensitive strains, via about 5 mg l−1 chitosan for an Aphanizomenon flos-aquae strain, a toxic P. agardhii strain and two Anabaena cylindrica cultures, to more than 8 mg l−1 chitosan for a Microcystis aeruginosa strain and another A. flos-aquae strain. Differences in sensitivity to chitosan might be related to polymeric substances that surround cyanobacteria. Rapid lysis of toxic strains is likely and when chitosan flocking and sinking of cyanobacteria is considered in lake restoration, flocculation efficacy studies should be complemented with investigation on the effects of chitosan on the cyanobacteria assemblage being targeted.

AB - Combining coagulant and ballast to remove cyanobacteria from the water column is a promising restoration technique to mitigate cyanobacterial nuisance in surface waters. The organic, biodegradable polymer chitosan has been promoted as a coagulant and is viewed as non-toxic. In this study, we show that chitosan may rapidly compromise membrane integrity and kill certain cyanobacteria leading to release of cell contents in the water. A strain of Cylindrospermopsis raciborskii and one strain of Planktothrix agardhii were most sensitive. A 1.3 h exposure to a low dose of 0.5 mg l−1 chitosan already almost completely killed these cultures resulting in release of cell contents. After 24 h, reductions in PSII efficiencies of all cyanobacteria tested were observed. EC50 values varied from around 0.5 mg l−1 chitosan for the two sensitive strains, via about 5 mg l−1 chitosan for an Aphanizomenon flos-aquae strain, a toxic P. agardhii strain and two Anabaena cylindrica cultures, to more than 8 mg l−1 chitosan for a Microcystis aeruginosa strain and another A. flos-aquae strain. Differences in sensitivity to chitosan might be related to polymeric substances that surround cyanobacteria. Rapid lysis of toxic strains is likely and when chitosan flocking and sinking of cyanobacteria is considered in lake restoration, flocculation efficacy studies should be complemented with investigation on the effects of chitosan on the cyanobacteria assemblage being targeted.

KW - Cell lysis

KW - Cell viability

KW - Cyanobacterial blooms

KW - Eutrophication

KW - Lake restoration

KW - Photosystem II efficiency

KW - international

U2 - 10.1016/j.watres.2017.04.020

DO - 10.1016/j.watres.2017.04.020

M3 - Article

VL - 118

SP - 121

EP - 130

JO - Water Research

T2 - Water Research

JF - Water Research

SN - 0043-1354

ER -

ID: 4295057