Clonal population of flucytosine-resistant Candida tropicalis from blood cultures, Paris, France

Marie Desnos-Ollivier; Stéphane Bretagne; Claire Bernède; Vincent Robert; Dorothée Raoux; Elisabeth Chachaty; Elisabeth Forget; Claire Lacroix; Françoise Dromer

doi:10.3201/eid1404.071083

Clonal population of flucytosine-resistant Candida tropicalis from blood cultures, Paris, France

Marie Desnos-Ollivier, Stéphane Bretagne, Claire Bernède, Vincent Robert, Dorothée Raoux, Elisabeth Chachaty, Elisabeth Forget, Claire Lacroix, Françoise Dromer,

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

46 Citations (Scopus)

Abstract

Candida tropicalis is a diploid ascomycetes yeast responsible for 4%-24% of candidemia. Resistance to flucytosine is rarely described for this species but was observed for 45 (35%) of 130 C. tropicalis isolates recovered from blood cultures in the Paris area in a 4-year survey. The aims of this study were to test the hypothesis that the flucytosine-resistant isolates could represent a subgroup and to determine the relationship between epidemiologic and genomic data. Epidemiologic data and gene sequences were analyzed, and molecular typing was performed. Our results suggest that a clone of flucytosine-resistant isolates, associated with malignancies and a lower mortality than that for other C. tropicalis isolates, is widespread in the Paris area. We propose the analysis of 2 polymorphic microsatellite markers coupled with URA3 sequencing to track the clone.

Original language	English
Pages (from-to)	557-65
Number of pages	9
Journal	Emerging Infectious Diseases
Volume	14
Issue number	4
DOIs	https://doi.org/10.3201/eid1404.071083
Publication status	Published - Apr 2008

Keywords

Antifungal Agents
Candida tropicalis
Candidiasis
Drug Resistance, Fungal
Flucytosine
France
Fungemia
Genotype
Humans
Microsatellite Repeats
Phenotype
Risk Factors
Time Factors

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title = "Clonal population of flucytosine-resistant Candida tropicalis from blood cultures, Paris, France",

abstract = "Candida tropicalis is a diploid ascomycetes yeast responsible for 4%-24% of candidemia. Resistance to flucytosine is rarely described for this species but was observed for 45 (35%) of 130 C. tropicalis isolates recovered from blood cultures in the Paris area in a 4-year survey. The aims of this study were to test the hypothesis that the flucytosine-resistant isolates could represent a subgroup and to determine the relationship between epidemiologic and genomic data. Epidemiologic data and gene sequences were analyzed, and molecular typing was performed. Our results suggest that a clone of flucytosine-resistant isolates, associated with malignancies and a lower mortality than that for other C. tropicalis isolates, is widespread in the Paris area. We propose the analysis of 2 polymorphic microsatellite markers coupled with URA3 sequencing to track the clone.",

keywords = "Antifungal Agents, Candida tropicalis, Candidiasis, Drug Resistance, Fungal, Flucytosine, France, Fungemia, Genotype, Humans, Microsatellite Repeats, Phenotype, Risk Factors, Time Factors",

author = "Marie Desnos-Ollivier and St{\'e}phane Bretagne and Claire Bern{\`e}de and Vincent Robert and Doroth{\'e}e Raoux and Elisabeth Chachaty and Elisabeth Forget and Claire Lacroix and Fran{\c c}oise Dromer",

year = "2008",

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doi = "10.3201/eid1404.071083",

language = "English",

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T1 - Clonal population of flucytosine-resistant Candida tropicalis from blood cultures, Paris, France

AU - Desnos-Ollivier, Marie

AU - Bretagne, Stéphane

AU - Bernède, Claire

AU - Robert, Vincent

AU - Raoux, Dorothée

AU - Chachaty, Elisabeth

AU - Forget, Elisabeth

AU - Lacroix, Claire

AU - Dromer, Françoise

PY - 2008/4

Y1 - 2008/4

N2 - Candida tropicalis is a diploid ascomycetes yeast responsible for 4%-24% of candidemia. Resistance to flucytosine is rarely described for this species but was observed for 45 (35%) of 130 C. tropicalis isolates recovered from blood cultures in the Paris area in a 4-year survey. The aims of this study were to test the hypothesis that the flucytosine-resistant isolates could represent a subgroup and to determine the relationship between epidemiologic and genomic data. Epidemiologic data and gene sequences were analyzed, and molecular typing was performed. Our results suggest that a clone of flucytosine-resistant isolates, associated with malignancies and a lower mortality than that for other C. tropicalis isolates, is widespread in the Paris area. We propose the analysis of 2 polymorphic microsatellite markers coupled with URA3 sequencing to track the clone.

AB - Candida tropicalis is a diploid ascomycetes yeast responsible for 4%-24% of candidemia. Resistance to flucytosine is rarely described for this species but was observed for 45 (35%) of 130 C. tropicalis isolates recovered from blood cultures in the Paris area in a 4-year survey. The aims of this study were to test the hypothesis that the flucytosine-resistant isolates could represent a subgroup and to determine the relationship between epidemiologic and genomic data. Epidemiologic data and gene sequences were analyzed, and molecular typing was performed. Our results suggest that a clone of flucytosine-resistant isolates, associated with malignancies and a lower mortality than that for other C. tropicalis isolates, is widespread in the Paris area. We propose the analysis of 2 polymorphic microsatellite markers coupled with URA3 sequencing to track the clone.

KW - Antifungal Agents

KW - Candida tropicalis

KW - Candidiasis

KW - Drug Resistance, Fungal

KW - Flucytosine

KW - France

KW - Fungemia

KW - Genotype

KW - Humans

KW - Microsatellite Repeats

KW - Phenotype

KW - Risk Factors

KW - Time Factors

U2 - 10.3201/eid1404.071083

DO - 10.3201/eid1404.071083

M3 - Article

C2 - 18394272

SN - 1080-6040

VL - 14

SP - 557

EP - 565

JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

IS - 4

ER -

Clonal population of flucytosine-resistant Candida tropicalis from blood cultures, Paris, France

Abstract

Keywords

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