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Collagen-based cell migration models in vitro and in vivo. / Wolf, K.; Alexander, S.; Schacht, V.; Coussens, L.M.; von Andrian, U.H.; van Rheenen, J.; Deryugina, E.; Friedl, P.

In: Seminars in Cell & Developmental Biology, Vol. 20, No. 8, 2009, p. 931-941.

Research output: Scientific - peer-reviewArticle

Harvard

Wolf, K, Alexander, S, Schacht, V, Coussens, LM, von Andrian, UH, van Rheenen, J, Deryugina, E & Friedl, P 2009, 'Collagen-based cell migration models in vitro and in vivo' Seminars in Cell & Developmental Biology, vol 20, no. 8, pp. 931-941. DOI: 10.1016/j.semcdb.2009.08.005

APA

Wolf, K., Alexander, S., Schacht, V., Coussens, L. M., von Andrian, U. H., van Rheenen, J., ... Friedl, P. (2009). Collagen-based cell migration models in vitro and in vivo. Seminars in Cell & Developmental Biology, 20(8), 931-941. DOI: 10.1016/j.semcdb.2009.08.005

Vancouver

Wolf K, Alexander S, Schacht V, Coussens LM, von Andrian UH, van Rheenen J et al. Collagen-based cell migration models in vitro and in vivo. Seminars in Cell & Developmental Biology. 2009;20(8):931-941. Available from, DOI: 10.1016/j.semcdb.2009.08.005

Author

Wolf, K.; Alexander, S.; Schacht, V.; Coussens, L.M.; von Andrian, U.H.; van Rheenen, J.; Deryugina, E.; Friedl, P. / Collagen-based cell migration models in vitro and in vivo.

In: Seminars in Cell & Developmental Biology, Vol. 20, No. 8, 2009, p. 931-941.

Research output: Scientific - peer-reviewArticle

BibTeX

@article{343bff117d5b4342a54f0aeeec697f61,
title = "Collagen-based cell migration models in vitro and in vivo",
abstract = "Fibrillar collagen is the most abundant extracellular matrix (ECM) constituent which maintains the structure of most interstitial tissues and organs, including skin, gut, and breast. Density and spatial alignments of the three-dimensional (3D) collagen architecture define mechanical tissue properties, i.e. stiffness and porosity, which guide or oppose cell migration and positioning in different contexts, such as morphogenesis, regeneration, immune response, and cancer progression. To reproduce interstitial cell movement in vitro with high in vivo fidelity, 3D collagen lattices are being reconstituted from extracted collagen monomers, resulting in the re-assembly of a fibrillar meshwork of defined porosity and stiffness. With a focus on tumor invasion studies, we here evaluate different in vitro collagen-based cell invasion models, employing either pepsinized or non-pepsinized collagen extracts, and compare their structure to connective tissue in vivo, including mouse dermis and mammary gland, chick chorioallantoic membrane (CAM), and human dermis. Using confocal reflection and two-photon-excited second harmonic generation (SHG) microscopy, we here show that, depending on the collagen source, in vitro models yield homogeneous fibrillar texture with a quite narrow range of pore size variation, whereas all in vivo scaffolds comprise a range from low- to high-density fibrillar networks and heterogeneous pore sizes within the same tissue. Future in-depth comparison of structure and physical properties between 3D ECM-based models in vitro and in vivo are mandatory to better understand the mechanisms and limits of interstitial cell movements in distinct tissue environments.",
author = "K. Wolf and S. Alexander and V. Schacht and L.M. Coussens and {von Andrian}, U.H. and {van Rheenen}, J. and E. Deryugina and P. Friedl",
note = "Reporting year: 2009 Metis note: S1084-9521(09)00161-x",
year = "2009",
doi = "10.1016/j.semcdb.2009.08.005",
volume = "20",
pages = "931--941",
journal = "Seminars in Cell and Developmental Biology",
issn = "1084-9521",
publisher = "Academic Press Inc.",
number = "8",

}

RIS

TY - JOUR

T1 - Collagen-based cell migration models in vitro and in vivo

AU - Wolf,K.

AU - Alexander,S.

AU - Schacht,V.

AU - Coussens,L.M.

AU - von Andrian,U.H.

AU - van Rheenen,J.

AU - Deryugina,E.

AU - Friedl,P.

N1 - Reporting year: 2009 Metis note: S1084-9521(09)00161-x

PY - 2009

Y1 - 2009

N2 - Fibrillar collagen is the most abundant extracellular matrix (ECM) constituent which maintains the structure of most interstitial tissues and organs, including skin, gut, and breast. Density and spatial alignments of the three-dimensional (3D) collagen architecture define mechanical tissue properties, i.e. stiffness and porosity, which guide or oppose cell migration and positioning in different contexts, such as morphogenesis, regeneration, immune response, and cancer progression. To reproduce interstitial cell movement in vitro with high in vivo fidelity, 3D collagen lattices are being reconstituted from extracted collagen monomers, resulting in the re-assembly of a fibrillar meshwork of defined porosity and stiffness. With a focus on tumor invasion studies, we here evaluate different in vitro collagen-based cell invasion models, employing either pepsinized or non-pepsinized collagen extracts, and compare their structure to connective tissue in vivo, including mouse dermis and mammary gland, chick chorioallantoic membrane (CAM), and human dermis. Using confocal reflection and two-photon-excited second harmonic generation (SHG) microscopy, we here show that, depending on the collagen source, in vitro models yield homogeneous fibrillar texture with a quite narrow range of pore size variation, whereas all in vivo scaffolds comprise a range from low- to high-density fibrillar networks and heterogeneous pore sizes within the same tissue. Future in-depth comparison of structure and physical properties between 3D ECM-based models in vitro and in vivo are mandatory to better understand the mechanisms and limits of interstitial cell movements in distinct tissue environments.

AB - Fibrillar collagen is the most abundant extracellular matrix (ECM) constituent which maintains the structure of most interstitial tissues and organs, including skin, gut, and breast. Density and spatial alignments of the three-dimensional (3D) collagen architecture define mechanical tissue properties, i.e. stiffness and porosity, which guide or oppose cell migration and positioning in different contexts, such as morphogenesis, regeneration, immune response, and cancer progression. To reproduce interstitial cell movement in vitro with high in vivo fidelity, 3D collagen lattices are being reconstituted from extracted collagen monomers, resulting in the re-assembly of a fibrillar meshwork of defined porosity and stiffness. With a focus on tumor invasion studies, we here evaluate different in vitro collagen-based cell invasion models, employing either pepsinized or non-pepsinized collagen extracts, and compare their structure to connective tissue in vivo, including mouse dermis and mammary gland, chick chorioallantoic membrane (CAM), and human dermis. Using confocal reflection and two-photon-excited second harmonic generation (SHG) microscopy, we here show that, depending on the collagen source, in vitro models yield homogeneous fibrillar texture with a quite narrow range of pore size variation, whereas all in vivo scaffolds comprise a range from low- to high-density fibrillar networks and heterogeneous pore sizes within the same tissue. Future in-depth comparison of structure and physical properties between 3D ECM-based models in vitro and in vivo are mandatory to better understand the mechanisms and limits of interstitial cell movements in distinct tissue environments.

U2 - 10.1016/j.semcdb.2009.08.005

DO - 10.1016/j.semcdb.2009.08.005

M3 - Article

VL - 20

SP - 931

EP - 941

JO - Seminars in Cell and Developmental Biology

T2 - Seminars in Cell and Developmental Biology

JF - Seminars in Cell and Developmental Biology

SN - 1084-9521

IS - 8

ER -

ID: 181507