Combined quantification of intracellular (phospho-)proteins and transcriptomics from fixed single cells

Jan P Gerlach, Jessie A G van Buggenum, Sabine E J Tanis, Mark Hogeweg, Branco M H Heuts, Mauro J Muraro, Lisa Elze, Francesca Rivello, Agata Rakszewska, Alexander van Oudenaarden, Wilhelm T S Huck, Hendrik G Stunnenberg, Klaas W Mulder

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

Environmental stimuli often lead to heterogeneous cellular responses and transcriptional output. We developed single-cell RNA and Immunodetection (RAID) to allow combined analysis of the transcriptome and intracellular (phospho-)proteins from fixed single cells. RAID successfully recapitulated differentiation-state changes at the protein and mRNA level in human keratinocytes. Furthermore, we show that differentiated keratinocytes that retain high phosphorylated FAK levels, a feature associated with stem cells, also express a selection of stem cell associated transcripts. Our data demonstrates that RAID allows investigation of heterogeneous cellular responses to environmental signals at the mRNA and phospho-proteome level.

Original languageEnglish
Pages (from-to)1469
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - 06 Feb 2019

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