Comparative analyses of the complete genome sequences of Pierce's disease and citrus variegated chlorosis strains of Xylella fastidiosa

M. A. Van Sluys; M. C. De Oliveira; C. B. Monteiro-Vitorello; C. Y. Miyaki; L. R. Furlan; L. E.A. Camargo; A. C.R. Da Silva; D. H. Moon; M. A. Takita; E. G.M. Lemos; M. A. Machado; M. I.T. Ferro; F. R. Da Silva; M. H.S. Goldman; G. H. Goldman; M. V.F. Lemos; H. El-Dorry; S. M. Tsai; H. Carrer; D. M. Carraro; R. C. De Oliveira; L. R. Nunes; W. J. Siqueira; L. L. Coutinho; E. T. Kimura; E. S. Ferro; R. Harakava; E. E. Kuramae; C. L. Marino; E. Giglioti; I. L. Abreu; L. M.C. Alves; A. M. Do Amaral; G. S. Baia; S. R. Blanco; M. S. Brito; F. S. Cannavan; A. V. Celestino; A. F. Da Cunha; R. C. Fenille; J. A. Ferro; E. F. Formighieri; L. T. Kishi; S. G. Leoni; A. R. Oliveira; V. E. Rosa; F. T. Sassaki; J. A.D. Sena; A. A. De Souza; D. Truffi; F. Tsukumo; G. M. Yanai; L. G. Zaros; E. L. Civerolo; A. J.G. Simpson; N. F. Almeida; J. C. Setubal; J. P. Kitajima

doi:10.1128/JB.185.3.1018-1026.2003

Comparative analyses of the complete genome sequences of Pierce's disease and citrus variegated chlorosis strains of Xylella fastidiosa

M. A. Van Sluys^*, M. C. De Oliveira, C. B. Monteiro-Vitorello, C. Y. Miyaki, L. R. Furlan, L. E.A. Camargo, A. C.R. Da Silva, D. H. Moon, M. A. Takita, E. G.M. Lemos, M. A. Machado, M. I.T. Ferro, F. R. Da Silva, M. H.S. Goldman, G. H. Goldman, M. V.F. Lemos, H. El-Dorry, S. M. Tsai, H. Carrer, D. M. CarraroR. C. De Oliveira, L. R. Nunes, W. J. Siqueira, L. L. Coutinho, E. T. Kimura, E. S. Ferro, R. Harakava, E. E. Kuramae, C. L. Marino, E. Giglioti, I. L. Abreu, L. M.C. Alves, A. M. Do Amaral, G. S. Baia, S. R. Blanco, M. S. Brito, F. S. Cannavan, A. V. Celestino, A. F. Da Cunha, R. C. Fenille, J. A. Ferro, E. F. Formighieri, L. T. Kishi, S. G. Leoni, A. R. Oliveira, V. E. Rosa, F. T. Sassaki, J. A.D. Sena, A. A. De Souza, D. Truffi, F. Tsukumo, G. M. Yanai, L. G. Zaros, E. L. Civerolo, A. J.G. Simpson, N. F. Almeida, J. C. Setubal, J. P. Kitajima

^*Corresponding author for this work

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283 Citations (Scopus)

Abstract

Xylella fastidiosa is a xylem-dwelling, insect-transmitted, gamma-proteobacterium that causes diseases in many plants, including grapevine, citrus, periwinkle, almond, oleander, and coffee. X. fastidiosa has an unusually broad host range, has an extensive geographical distribution throughout the American continent, and induces diverse disease phenotypes. Previous molecular analyses indicated three distinct groups of X. fastidiosa isolates that were expected to be genetically divergent. Here we report the genome sequence of X. fastidiosa (Temecula strain), isolated from a naturally infected grapevine with Pierce's disease (PD) in a wine-grapegrowing region of California. Comparative analyses with a previously sequenced X. fastidiosa strain responsible for citrus variegated chlorosis (CVC) revealed that 98% of the PD X. fastidiosa Temecula genes are shared with the CVC X. fastidiosa strain 9a5c genes. Furthermore, the average amino acid identity of the open reading frames in the strains is 95.7%. Genomic differences are limited to phage-associated chromosomal rearrangements and deletions that also account for the strain-specific genes present in each genome. Genomic islands, one in each genome, were identified, and their presence in other X. fastidiosa strains was analyzed. We conclude that these two organisms have identical metabolic functions and are likely to use a common set of genes in plant colonization and pathogenesis, permitting convergence of functional genomic strategies.

Original language	English
Pages (from-to)	1018-1026
Number of pages	9
Journal	Journal of Bacteriology
Volume	185
Issue number	3
DOIs	https://doi.org/10.1128/JB.185.3.1018-1026.2003
Publication status	Published - Feb 2003

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10.1128/JB.185.3.1018-1026.2003

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Van Sluys, M. A., De Oliveira, M. C., Monteiro-Vitorello, C. B., Miyaki, C. Y., Furlan, L. R., Camargo, L. E. A., Da Silva, A. C. R., Moon, D. H., Takita, M. A., Lemos, E. G. M., Machado, M. A., Ferro, M. I. T., Da Silva, F. R., Goldman, M. H. S., Goldman, G. H., Lemos, M. V. F., El-Dorry, H., Tsai, S. M., Carrer, H., ... Kitajima, J. P. (2003). Comparative analyses of the complete genome sequences of Pierce's disease and citrus variegated chlorosis strains of Xylella fastidiosa. Journal of Bacteriology, 185(3), 1018-1026. https://doi.org/10.1128/JB.185.3.1018-1026.2003

@article{592d2630567c44d29208ee847c0ea3ff,

title = "Comparative analyses of the complete genome sequences of Pierce's disease and citrus variegated chlorosis strains of Xylella fastidiosa",

abstract = "Xylella fastidiosa is a xylem-dwelling, insect-transmitted, gamma-proteobacterium that causes diseases in many plants, including grapevine, citrus, periwinkle, almond, oleander, and coffee. X. fastidiosa has an unusually broad host range, has an extensive geographical distribution throughout the American continent, and induces diverse disease phenotypes. Previous molecular analyses indicated three distinct groups of X. fastidiosa isolates that were expected to be genetically divergent. Here we report the genome sequence of X. fastidiosa (Temecula strain), isolated from a naturally infected grapevine with Pierce's disease (PD) in a wine-grapegrowing region of California. Comparative analyses with a previously sequenced X. fastidiosa strain responsible for citrus variegated chlorosis (CVC) revealed that 98% of the PD X. fastidiosa Temecula genes are shared with the CVC X. fastidiosa strain 9a5c genes. Furthermore, the average amino acid identity of the open reading frames in the strains is 95.7%. Genomic differences are limited to phage-associated chromosomal rearrangements and deletions that also account for the strain-specific genes present in each genome. Genomic islands, one in each genome, were identified, and their presence in other X. fastidiosa strains was analyzed. We conclude that these two organisms have identical metabolic functions and are likely to use a common set of genes in plant colonization and pathogenesis, permitting convergence of functional genomic strategies.",

author = "{Van Sluys}, {M. A.} and {De Oliveira}, {M. C.} and Monteiro-Vitorello, {C. B.} and Miyaki, {C. Y.} and Furlan, {L. R.} and Camargo, {L. E.A.} and {Da Silva}, {A. C.R.} and Moon, {D. H.} and Takita, {M. A.} and Lemos, {E. G.M.} and Machado, {M. A.} and Ferro, {M. I.T.} and {Da Silva}, {F. R.} and Goldman, {M. H.S.} and Goldman, {G. H.} and Lemos, {M. V.F.} and H. El-Dorry and Tsai, {S. M.} and H. Carrer and Carraro, {D. M.} and {De Oliveira}, {R. C.} and Nunes, {L. R.} and Siqueira, {W. J.} and Coutinho, {L. L.} and Kimura, {E. T.} and Ferro, {E. S.} and R. Harakava and Kuramae, {E. E.} and Marino, {C. L.} and E. Giglioti and Abreu, {I. L.} and Alves, {L. M.C.} and {Do Amaral}, {A. M.} and Baia, {G. S.} and Blanco, {S. R.} and Brito, {M. S.} and Cannavan, {F. S.} and Celestino, {A. V.} and {Da Cunha}, {A. F.} and Fenille, {R. C.} and Ferro, {J. A.} and Formighieri, {E. F.} and Kishi, {L. T.} and Leoni, {S. G.} and Oliveira, {A. R.} and Rosa, {V. E.} and Sassaki, {F. T.} and Sena, {J. A.D.} and {De Souza}, {A. A.} and D. Truffi and F. Tsukumo and Yanai, {G. M.} and Zaros, {L. G.} and Civerolo, {E. L.} and Simpson, {A. J.G.} and Almeida, {N. F.} and Setubal, {J. C.} and Kitajima, {J. P.}",

year = "2003",

month = feb,

doi = "10.1128/JB.185.3.1018-1026.2003",

language = "English",

volume = "185",

pages = "1018--1026",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "3",

}

Van Sluys, MA, De Oliveira, MC, Monteiro-Vitorello, CB, Miyaki, CY, Furlan, LR, Camargo, LEA, Da Silva, ACR, Moon, DH, Takita, MA, Lemos, EGM, Machado, MA, Ferro, MIT, Da Silva, FR, Goldman, MHS, Goldman, GH, Lemos, MVF, El-Dorry, H, Tsai, SM, Carrer, H, Carraro, DM, De Oliveira, RC, Nunes, LR, Siqueira, WJ, Coutinho, LL, Kimura, ET, Ferro, ES, Harakava, R, Kuramae, EE, Marino, CL, Giglioti, E, Abreu, IL, Alves, LMC, Do Amaral, AM, Baia, GS, Blanco, SR, Brito, MS, Cannavan, FS, Celestino, AV, Da Cunha, AF, Fenille, RC, Ferro, JA, Formighieri, EF, Kishi, LT, Leoni, SG, Oliveira, AR, Rosa, VE, Sassaki, FT, Sena, JAD, De Souza, AA, Truffi, D, Tsukumo, F, Yanai, GM, Zaros, LG, Civerolo, EL, Simpson, AJG, Almeida, NF, Setubal, JC & Kitajima, JP 2003, 'Comparative analyses of the complete genome sequences of Pierce's disease and citrus variegated chlorosis strains of Xylella fastidiosa', Journal of Bacteriology, vol. 185, no. 3, pp. 1018-1026. https://doi.org/10.1128/JB.185.3.1018-1026.2003

Comparative analyses of the complete genome sequences of Pierce's disease and citrus variegated chlorosis strains of Xylella fastidiosa. / Van Sluys, M. A.; De Oliveira, M. C.; Monteiro-Vitorello, C. B. et al.
In: Journal of Bacteriology, Vol. 185, No. 3, 02.2003, p. 1018-1026.

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

TY - JOUR

T1 - Comparative analyses of the complete genome sequences of Pierce's disease and citrus variegated chlorosis strains of Xylella fastidiosa

AU - Van Sluys, M. A.

AU - De Oliveira, M. C.

AU - Monteiro-Vitorello, C. B.

AU - Miyaki, C. Y.

AU - Furlan, L. R.

AU - Camargo, L. E.A.

AU - Da Silva, A. C.R.

AU - Moon, D. H.

AU - Takita, M. A.

AU - Lemos, E. G.M.

AU - Machado, M. A.

AU - Ferro, M. I.T.

AU - Da Silva, F. R.

AU - Goldman, M. H.S.

AU - Goldman, G. H.

AU - Lemos, M. V.F.

AU - El-Dorry, H.

AU - Tsai, S. M.

AU - Carrer, H.

AU - Carraro, D. M.

AU - De Oliveira, R. C.

AU - Nunes, L. R.

AU - Siqueira, W. J.

AU - Coutinho, L. L.

AU - Kimura, E. T.

AU - Ferro, E. S.

AU - Harakava, R.

AU - Kuramae, E. E.

AU - Marino, C. L.

AU - Giglioti, E.

AU - Abreu, I. L.

AU - Alves, L. M.C.

AU - Do Amaral, A. M.

AU - Baia, G. S.

AU - Blanco, S. R.

AU - Brito, M. S.

AU - Cannavan, F. S.

AU - Celestino, A. V.

AU - Da Cunha, A. F.

AU - Fenille, R. C.

AU - Ferro, J. A.

AU - Formighieri, E. F.

AU - Kishi, L. T.

AU - Leoni, S. G.

AU - Oliveira, A. R.

AU - Rosa, V. E.

AU - Sassaki, F. T.

AU - Sena, J. A.D.

AU - De Souza, A. A.

AU - Truffi, D.

AU - Tsukumo, F.

AU - Yanai, G. M.

AU - Zaros, L. G.

AU - Civerolo, E. L.

AU - Simpson, A. J.G.

AU - Almeida, N. F.

AU - Setubal, J. C.

AU - Kitajima, J. P.

PY - 2003/2

Y1 - 2003/2

N2 - Xylella fastidiosa is a xylem-dwelling, insect-transmitted, gamma-proteobacterium that causes diseases in many plants, including grapevine, citrus, periwinkle, almond, oleander, and coffee. X. fastidiosa has an unusually broad host range, has an extensive geographical distribution throughout the American continent, and induces diverse disease phenotypes. Previous molecular analyses indicated three distinct groups of X. fastidiosa isolates that were expected to be genetically divergent. Here we report the genome sequence of X. fastidiosa (Temecula strain), isolated from a naturally infected grapevine with Pierce's disease (PD) in a wine-grapegrowing region of California. Comparative analyses with a previously sequenced X. fastidiosa strain responsible for citrus variegated chlorosis (CVC) revealed that 98% of the PD X. fastidiosa Temecula genes are shared with the CVC X. fastidiosa strain 9a5c genes. Furthermore, the average amino acid identity of the open reading frames in the strains is 95.7%. Genomic differences are limited to phage-associated chromosomal rearrangements and deletions that also account for the strain-specific genes present in each genome. Genomic islands, one in each genome, were identified, and their presence in other X. fastidiosa strains was analyzed. We conclude that these two organisms have identical metabolic functions and are likely to use a common set of genes in plant colonization and pathogenesis, permitting convergence of functional genomic strategies.

AB - Xylella fastidiosa is a xylem-dwelling, insect-transmitted, gamma-proteobacterium that causes diseases in many plants, including grapevine, citrus, periwinkle, almond, oleander, and coffee. X. fastidiosa has an unusually broad host range, has an extensive geographical distribution throughout the American continent, and induces diverse disease phenotypes. Previous molecular analyses indicated three distinct groups of X. fastidiosa isolates that were expected to be genetically divergent. Here we report the genome sequence of X. fastidiosa (Temecula strain), isolated from a naturally infected grapevine with Pierce's disease (PD) in a wine-grapegrowing region of California. Comparative analyses with a previously sequenced X. fastidiosa strain responsible for citrus variegated chlorosis (CVC) revealed that 98% of the PD X. fastidiosa Temecula genes are shared with the CVC X. fastidiosa strain 9a5c genes. Furthermore, the average amino acid identity of the open reading frames in the strains is 95.7%. Genomic differences are limited to phage-associated chromosomal rearrangements and deletions that also account for the strain-specific genes present in each genome. Genomic islands, one in each genome, were identified, and their presence in other X. fastidiosa strains was analyzed. We conclude that these two organisms have identical metabolic functions and are likely to use a common set of genes in plant colonization and pathogenesis, permitting convergence of functional genomic strategies.

UR - http://www.scopus.com/inward/record.url?scp=0037310805&partnerID=8YFLogxK

U2 - 10.1128/JB.185.3.1018-1026.2003

DO - 10.1128/JB.185.3.1018-1026.2003

M3 - Article

C2 - 12533478

AN - SCOPUS:0037310805

SN - 0021-9193

VL - 185

SP - 1018

EP - 1026

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 3

ER -

Comparative analyses of the complete genome sequences of Pierce's disease and citrus variegated chlorosis strains of Xylella fastidiosa

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