Competition of a parathion-hydrolyzing Flavobacterium with bacteria from ditch water in carbon-, nitrate- and phosphate-limited continuous cultures

TY - JOUR

T1 - Competition of a parathion-hydrolyzing Flavobacterium with bacteria from ditch water in carbon-, nitrate- and phosphate-limited continuous cultures

AU - Sprenger, W.

AU - Dijkstra, A.

AU - Zwart, G.

AU - Van Agterveld, M.P.

AU - Van Noort, P.C.M.

AU - Parsons, J.R.

N1 - Reporting year: 2003 Metis note: 3119; CL; MWE; file:///L:/Endnotedatabases/NIOOPUB/pdfs/Pdfs2003/Sprenger_ea_3119.pdf

PY - 2003

Y1 - 2003

N2 - The effect of competition for macroelements with bacteria from ditch water on the parathion-hydrolyzing Flavobacterium sp. ATCC 27551 (FB) was investigated within mixed continuous cultures under carbon-, nitrate- or phosphate-limited conditions. The high initial rate of parathion hydrolysis decreased rapidly in all cultures due to the loss of strain FB. Addition of 2-isopropyl-6-methyl-4-pyrimidinol (a selective source of carbon, nitrogen and energy for FB) to one nitrate- and carbon-limited chemostat caused a 20-fold increase in parathion-hydrolyzing activity compared to unamended control cultures and retention of FB. The presence of the parathion hydrolase-encoding gene could be demonstrated by a newly developed PCR detection method in all FB cultures during most of the cultivation period. These results suggest that competition effects cause the pesticide-degrading capacity of microbial communities depending on their frequency of exposure to the pesticide compounds. [KEYWORDS: Organophosphorus; Pesticide; Biodegradation; Nutrient; Competition; Chemostat]

AB - The effect of competition for macroelements with bacteria from ditch water on the parathion-hydrolyzing Flavobacterium sp. ATCC 27551 (FB) was investigated within mixed continuous cultures under carbon-, nitrate- or phosphate-limited conditions. The high initial rate of parathion hydrolysis decreased rapidly in all cultures due to the loss of strain FB. Addition of 2-isopropyl-6-methyl-4-pyrimidinol (a selective source of carbon, nitrogen and energy for FB) to one nitrate- and carbon-limited chemostat caused a 20-fold increase in parathion-hydrolyzing activity compared to unamended control cultures and retention of FB. The presence of the parathion hydrolase-encoding gene could be demonstrated by a newly developed PCR detection method in all FB cultures during most of the cultivation period. These results suggest that competition effects cause the pesticide-degrading capacity of microbial communities depending on their frequency of exposure to the pesticide compounds. [KEYWORDS: Organophosphorus; Pesticide; Biodegradation; Nutrient; Competition; Chemostat]

U2 - 10.1016/S0168-6496(02)00415-4

DO - 10.1016/S0168-6496(02)00415-4

M3 - Article

SN - 0168-6496

VL - 43

SP - 45

EP - 53

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

IS - 1

ER -