TY - JOUR
T1 - Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice
AU - Gargareta, Vasiliki-Ilya
AU - Reuschenbach, Josefine
AU - Siems, Sophie B
AU - Sun, Ting
AU - Piepkorn, Lars
AU - Mangana, Carolina
AU - Späte, Erik
AU - Goebbels, Sandra
AU - Huitinga, Inge
AU - Möbius, Wiebke
AU - Nave, Klaus-Armin
AU - Jahn, Olaf
AU - Werner, Hauke B
N1 - © 2022, Gargareta et al.
PY - 2022/5/11
Y1 - 2022/5/11
N2 - Human myelin disorders are commonly studied in mouse models. Since both clades evolutionarily diverged approximately 85 million years ago, it is critical to know to what extent the myelin protein composition has remained similar. Here, we use quantitative proteomics to analyze myelin purified from human white matter and find that the relative abundance of the structural myelin proteins PLP, MBP, CNP, and SEPTIN8 correlates well with that in C57Bl/6N mice. Conversely, multiple other proteins were identified exclusively or predominantly in human or mouse myelin. This is exemplified by peripheral myelin protein 2 (PMP2), which was specific to human central nervous system myelin, while tetraspanin-2 (TSPAN2) and connexin-29 (CX29/GJC3) were confined to mouse myelin. Assessing published scRNA-seq-datasets, human and mouse oligodendrocytes display well-correlating transcriptome profiles but divergent expression of distinct genes, including Pmp2, Tspan2, and Gjc3. A searchable web interface is accessible via www.mpinat.mpg.de/myelin. Species-dependent diversity of oligodendroglial mRNA expression and myelin protein composition can be informative when translating from mouse models to humans.
AB - Human myelin disorders are commonly studied in mouse models. Since both clades evolutionarily diverged approximately 85 million years ago, it is critical to know to what extent the myelin protein composition has remained similar. Here, we use quantitative proteomics to analyze myelin purified from human white matter and find that the relative abundance of the structural myelin proteins PLP, MBP, CNP, and SEPTIN8 correlates well with that in C57Bl/6N mice. Conversely, multiple other proteins were identified exclusively or predominantly in human or mouse myelin. This is exemplified by peripheral myelin protein 2 (PMP2), which was specific to human central nervous system myelin, while tetraspanin-2 (TSPAN2) and connexin-29 (CX29/GJC3) were confined to mouse myelin. Assessing published scRNA-seq-datasets, human and mouse oligodendrocytes display well-correlating transcriptome profiles but divergent expression of distinct genes, including Pmp2, Tspan2, and Gjc3. A searchable web interface is accessible via www.mpinat.mpg.de/myelin. Species-dependent diversity of oligodendroglial mRNA expression and myelin protein composition can be informative when translating from mouse models to humans.
U2 - 10.7554/eLife.77019
DO - 10.7554/eLife.77019
M3 - Article
C2 - 35543322
SN - 2050-084X
VL - 11
JO - eLife
JF - eLife
M1 - e77019
ER -