Controlled gene expression in primary Lgr5 organoid cultures

B.K. Koo, D.E. Stange, T. Sato, W. Karthaus, H.F. Farin, M. Huch, J.H. van Es, H. Clevers

Research output: Contribution to journal/periodicalArticleScientificpeer-review

247 Citations (Scopus)


The study of gene function in endodermal epithelia such as of stomach, small intestine and colon relies heavily on transgenic approaches. Establishing such animal models is laborious, expensive and time-consuming. We present here a method based on Cre recombinase-inducible retrovirus vectors that allows the conditional manipulation of gene expression in primary mouse organoid culture systems.
Original languageEnglish
Pages (from-to)81-83
JournalNature Methods
Issue number1
Publication statusPublished - 2011


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