Controlled gene expression in primary Lgr5 organoid cultures

TY - JOUR

T1 - Controlled gene expression in primary Lgr5 organoid cultures

AU - Koo, B.K.

AU - Stange, D.E.

AU - Sato, T.

AU - Karthaus, W.

AU - Farin, H.F.

AU - Huch, M.

AU - van Es, J.H.

AU - Clevers, H.

N1 - Reporting year: 2011

PY - 2011

Y1 - 2011

N2 - The study of gene function in endodermal epithelia such as of stomach, small intestine and colon relies heavily on transgenic approaches. Establishing such animal models is laborious, expensive and time-consuming. We present here a method based on Cre recombinase-inducible retrovirus vectors that allows the conditional manipulation of gene expression in primary mouse organoid culture systems.

AB - The study of gene function in endodermal epithelia such as of stomach, small intestine and colon relies heavily on transgenic approaches. Establishing such animal models is laborious, expensive and time-consuming. We present here a method based on Cre recombinase-inducible retrovirus vectors that allows the conditional manipulation of gene expression in primary mouse organoid culture systems.

U2 - 10.1038/NMETH.1802

DO - 10.1038/NMETH.1802

M3 - Article

SN - 1548-7091

VL - 9

SP - 81

EP - 83

JO - Nature Methods

JF - Nature Methods

IS - 1

ER -