Controlled Release of RNAi Molecules by Tunable Supramolecular Hydrogel Carriers

Maarten H Bakker; Eva van Rooij; Patricia Y W Dankers

doi:10.1002/asia.201800582

Controlled Release of RNAi Molecules by Tunable Supramolecular Hydrogel Carriers

Maarten H Bakker, Eva van Rooij, Patricia Y W Dankers

Hubrecht Institute

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

21 Citations (Scopus)

Abstract

Local, sustained release and presentation of RNAi therapeutics can be achieved with hydrogel delivery systems. Here we show the development of a supramolecular hydrogel into a local RNAi delivery system. By careful material design, two simple but effective strategies are introduced to obtain controlled release of two classes of RNAi therapeutics, that is, microRNA and antimiR. It was shown that the release of microRNA could be regulated using cholesterol-modification for interaction with the supramolecular hydrogel. Non-modified antimiR release could be controlled via supramolecular introduction of positively charged additive molecules into the supramolecular hydrogel. In this way, either the cholesterol-modification on the drug or the charge introduction into the hydrogel provides handles for controlled RNAi therapy.

Original language	English
Pages (from-to)	3501-3508
Number of pages	8
Journal	Chemistry - An Asian Journal
Volume	13
Issue number	22
DOIs	https://doi.org/10.1002/asia.201800582
Publication status	Published - 16 Nov 2018

Keywords

Antagomirs/chemistry
Cholesterol/chemistry
Drug Carriers/chemistry
Drug Liberation
Fluorescence Recovery After Photobleaching
HEK293 Cells
Humans
Hydrogels/chemistry
Hydrogen-Ion Concentration
Polyethylene Glycols/chemistry
RNA Interference
RNA, Small Interfering/chemistry
Rheology
Viscosity

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10.1002/asia.201800582

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title = "Controlled Release of RNAi Molecules by Tunable Supramolecular Hydrogel Carriers",

abstract = "Local, sustained release and presentation of RNAi therapeutics can be achieved with hydrogel delivery systems. Here we show the development of a supramolecular hydrogel into a local RNAi delivery system. By careful material design, two simple but effective strategies are introduced to obtain controlled release of two classes of RNAi therapeutics, that is, microRNA and antimiR. It was shown that the release of microRNA could be regulated using cholesterol-modification for interaction with the supramolecular hydrogel. Non-modified antimiR release could be controlled via supramolecular introduction of positively charged additive molecules into the supramolecular hydrogel. In this way, either the cholesterol-modification on the drug or the charge introduction into the hydrogel provides handles for controlled RNAi therapy.",

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author = "Bakker, {Maarten H} and {van Rooij}, Eva and Dankers, {Patricia Y W}",

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doi = "10.1002/asia.201800582",

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AU - Bakker, Maarten H

AU - van Rooij, Eva

AU - Dankers, Patricia Y W

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Y1 - 2018/11/16

N2 - Local, sustained release and presentation of RNAi therapeutics can be achieved with hydrogel delivery systems. Here we show the development of a supramolecular hydrogel into a local RNAi delivery system. By careful material design, two simple but effective strategies are introduced to obtain controlled release of two classes of RNAi therapeutics, that is, microRNA and antimiR. It was shown that the release of microRNA could be regulated using cholesterol-modification for interaction with the supramolecular hydrogel. Non-modified antimiR release could be controlled via supramolecular introduction of positively charged additive molecules into the supramolecular hydrogel. In this way, either the cholesterol-modification on the drug or the charge introduction into the hydrogel provides handles for controlled RNAi therapy.

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KW - Antagomirs/chemistry

KW - Cholesterol/chemistry

KW - Drug Carriers/chemistry

KW - Drug Liberation

KW - Fluorescence Recovery After Photobleaching

KW - HEK293 Cells

KW - Humans

KW - Hydrogels/chemistry

KW - Hydrogen-Ion Concentration

KW - Polyethylene Glycols/chemistry

KW - RNA Interference

KW - RNA, Small Interfering/chemistry

KW - Rheology

KW - Viscosity

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EP - 3508

JO - Chemistry - An Asian Journal

JF - Chemistry - An Asian Journal

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ER -

Controlled Release of RNAi Molecules by Tunable Supramolecular Hydrogel Carriers

Abstract

Keywords

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