CRISPR-Cas Tools and Their Application in Genetic Engineering of Human Stem Cells and Organoids

Delilah Hendriks, Hans Clevers, Benedetta Artegiani

Research output: Contribution to journal/periodicalBook/Film/Article reviewScientific

Abstract

CRISPR-Cas technology has revolutionized biological research and holds great therapeutic potential. Here, we review CRISPR-Cas systems and their latest developments with an emphasis on application to human cells. We also discuss how different CRISPR-based strategies can be used to accomplish a particular genome engineering goal. We then review how different CRISPR tools have been used in genome engineering of human stem cells in vitro, covering both the pluripotent (iPSC/ESC) and somatic adult stem cell fields and, in particular, 3D organoid cultures. Finally, we discuss the progress and challenges associated with CRISPR-based genome editing of human stem cells for therapeutic use.

Original languageEnglish
Pages (from-to)705-731
Number of pages27
JournalCell Stem Cell
Volume27
Issue number5
DOIs
Publication statusPublished - 05 Nov 2020

Fingerprint Dive into the research topics of 'CRISPR-Cas Tools and Their Application in Genetic Engineering of Human Stem Cells and Organoids'. Together they form a unique fingerprint.

Cite this