CRISPR/Cas 9 genome editing and its applications in organoids

Else Driehuis, Hans Clevers

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

Organoids are three-dimensional (3D) structures derived from adult or embryonic stem cells that maintain many structural and functional features of their respective organ. Recently, genome editing based on the bacterial defense mechanism CRISPR/Cas9 has emerged as an easily applicable and reliable laboratory tool. Combining organoids and CRISPR/Cas9 creates exciting new opportunities to study organ development and human disease in vitro. The potential applications of CRISPR in organoids are only beginning to be explored.

Original languageEnglish
Pages (from-to)G257-G265
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume312
Issue number3
DOIs
Publication statusPublished - 01 Mar 2017

Keywords

  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Gene Editing
  • Humans
  • Organoids
  • Journal Article
  • Review

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