Database establishment for the secondary fungal DNA barcode translational elongation factor 1 alpha (TEF1 alpha)

Wieland Meyer, Laszlo Irinyi, Minh Thuy Vi Hoang, Vincent Robert, Dea Garcia-Hermoso, Marie Desnos-Ollivier, Chompoonek Yurayart, Chi-Ching Tsang, Chun-Yi Lee, Patrick C. Y. Woo, Ivan Mikhailovich Pchelin, Silke Uhrlass, Pietro Nenoff, Ariya Chindamporn, Sharon Chen, Paul D. N. Hebert, Tania C. Sorrell, Catriona Halliday, Ian Arthur, Maria Luiza MorettiCelia Maria de Almeida Soares, Mauro de Medeiros Muniz, Rosely Maria Zancope-Oliveira, Fundacao Oswaldo Cruz, Analy Salles de Azevedo Melo, Arnaldo L. Colombo, Angela Satie Nishikaku, Marijke Hendrickx, Dirk Stubbe, Anne-Cecile Normand, Renaud Piarroux, Stephane Ranque, Francoise Dromer, Michael Arabatzis, Aristea Velegraki, Gianluigi Cardinali, Laura Rosio Castanon, Maria Lucia Taylor, Conchita Toriello, Sybren de Hoog, Celia Pais, Wilhelm de Beer, Marieka Gryzenhout, Josep Guarro, Jose F. Cano-Lira, Barbara Robbertse, Conrad Schoch

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

With new or emerging fungal infections, human/animal fungal pathogens are a growing threat worldwide. Current diagnostic tools are slow, non-specific at the species and subspecies levels, and require specific morphological expertise to accurately identify pathogens from pure cultures. DNA barcodes are easily amplified, universal, short species-specific DNA sequences, which enable rapid identification by comparison with a well-curated reference sequence collection. The primary fungal DNA barcode, ITS region, was introduced in 2012 and is now routinely used in diagnostic laboratories. However, the ITS region only accurately identifies around 75% of all medically relevant fungal species, which has prompted the development of a secondary barcode to increase the resolution power and suitability of DNA barcoding for fungal disease diagnostics. The translational elongation factor 1α (TEF1α) was selected in 2015 as a secondary fungal DNA barcode, but has not been implemented into practice, due to the absence of a reference database. Here, we have established a quality-controlled reference database for the secondary barcode which together with the ISHAM-ITS database, forms the "ISHAM BARCODE DATABASE", available online at: http://its.mycologylab.org/. We encourage the mycology community for active contributions. The application of a dual DNA barcoding system enables accurate identification of all clinically important fungal pathogens.

Original languageEnglish
Pages (from-to)160-169
JournalGenome
Volume62
Issue number3
Early online date22 Nov 2018
DOIs
Publication statusPublished - Mar 2019

Keywords

  • fungal DNA barcoding
  • secondary fungal DNA barcode database
  • translational elongation factor 1 alpha

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