Deletion of the serotonin transporter in rats disturbs serotonin homeostasis without impairing liver regeneration

TY - JOUR

T1 - Deletion of the serotonin transporter in rats disturbs serotonin homeostasis without impairing liver regeneration

AU - Matondo, R.B.

AU - Punt, C.

AU - Homberg, J.R.

AU - Toussaint, M.J.

AU - Kisjes, R.

AU - Korporaal, S.J.

AU - Akkerman, J.W.

AU - Cuppen, E.

AU - de Bruin, A.

N1 - Reporting year: 2009 Metis note: 90709.2008

PY - 2009

Y1 - 2009

N2 - The serotonin transporter is implicated in the uptake of the vasoconstrictor serotonin from the circulation into the platelets, where 95% of all blood serotonin is stored and released in response to vascular injury. In vivo studies indicated that platelet-derived serotonin mediates liver regeneration after partial hepatectomy. We have recently generated serotonin transporter knockout rats and demonstrated that their platelets were almost completely depleted of serotonin. Here we show that these rats exhibit impaired hemostasis and contain about 1-6% of wild-type serotonin levels in the blood. Despite the marked reduction of serotonin levels in blood and platelets, efficient liver regeneration and collagen-induced platelet aggregation occur in rats lacking the serotonin transporter. These results provide evidence that liver regeneration is not dependent on the release of serotonin from platelets. Our findings indicate that very low levels of serotonin in blood are sufficient for liver regeneration.

AB - The serotonin transporter is implicated in the uptake of the vasoconstrictor serotonin from the circulation into the platelets, where 95% of all blood serotonin is stored and released in response to vascular injury. In vivo studies indicated that platelet-derived serotonin mediates liver regeneration after partial hepatectomy. We have recently generated serotonin transporter knockout rats and demonstrated that their platelets were almost completely depleted of serotonin. Here we show that these rats exhibit impaired hemostasis and contain about 1-6% of wild-type serotonin levels in the blood. Despite the marked reduction of serotonin levels in blood and platelets, efficient liver regeneration and collagen-induced platelet aggregation occur in rats lacking the serotonin transporter. These results provide evidence that liver regeneration is not dependent on the release of serotonin from platelets. Our findings indicate that very low levels of serotonin in blood are sufficient for liver regeneration.

U2 - 10.1152/ajpgi.90709.2008

DO - 10.1152/ajpgi.90709.2008

M3 - Article

SN - 0193-1857

VL - 296

SP - 963

EP - 968

JO - American Journal of Physiology - Gastrointestinal and Liver Physiology

JF - American Journal of Physiology - Gastrointestinal and Liver Physiology

IS - 4

ER -